EDAG protects hematopoietic stem cells from damage induced by chronic inflammation
10.7644/j.issn.1674-9960.2024.08.006
- VernacularTitle:人类红系分化相关基因对慢性炎症诱导的造血干细胞损伤的保护作用
- Author:
Siyu LI
1
,
2
;
Ke ZHAO
;
Yunqiang WU
;
Zhichun LÜ
;
Huiying SUN
;
Xiongwei ZHAO
;
Yang XUE
;
Huiying GAO
;
Shensi XIANG
;
Changyan LI
Author Information
1. 安徽医科大学基础医学院,合肥 230032
2. 军事科学院军事医学研究院,北京 100850
- Keywords:
erythroid differentiation associated gene;
chronic inflammation;
hematopoietic stem/progenitor cells;
exhaustion;
aging
- From:
Military Medical Sciences
2024;48(8):594-600
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the effect of erythroid differentiation associated gene(EDAG)on hematopoietic stem/progenitor cells(HSPCs)under chronic inflammation.Methods In this study,EDAG-/-and wild type(WT)mice were divided into the experiment group and control group.An infectious chronic inflammation model was established via multiple intraperitoneal injections of Listeria monocytogenes(LM),while a sterile chronic inflammation model was generated via multiple intraperitoneal injections of polyinosinic-polycytidylic acid[Poly(I∶C)].The effect of EDAG on HSPCs was explored under chronic inflammation conditions.Results In the LM repeated infection model,EDAG deletion led to a decrease in HSPCs and long-term hematopoietic stem cells(LT-HSCs)in mice as well as a significant bias towards myeloid differentiation in peripheral blood.Similarly,EDAG knockout also resulted in reduced numbers of HSPCs and decreased colony-forming ability in aseptic chronic inflammation models.Conclusion EDAG deficiency accelerates HSPC depletion in young mice under chronic inflammation,indicating strong protection of EDAG against HSPC damage induced by chronic inflammation.
