Glutathione peroxidase 3-modified mesenchymal stem cells attenuate radiation-induced lung injury by reducing oxidative damage
10.7644/j.issn.1674-9960.2024.07.002
- VernacularTitle:谷胱甘肽过氧化物酶3修饰的间充质干细胞通过降低氧化损伤减轻放射性肺损伤
- Author:
Rui ZHAI
1
,
2
;
Fumin TAI
;
Kexin DING
;
Changhui GE
;
Xiaofei ZHENG
;
Hanjiang FU
Author Information
1. 安徽医科大学基础医学院,合肥 230032
2. 军事科学院军事医学研究院,放射生物学北京市重点实验室,北京 100850
- Keywords:
glutathione peroxidase 3;
mesenchymal stem cells;
oxidative damage;
radiation-induced lung injury;
8-hydroxy guanine
- From:
Military Medical Sciences
2024;48(7):487-494
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the protective effect and mechanism of glutathione peroxidase 3-(GPx3)modified mesenchymal stem cells(MSC)against radiation-induced lung injury(RILI).Methods GPx3-modified MSCs were injected into the tail vein of mice whose lungs were irradiated with 20 Gy.Lung tissues were collected and sections were stained to observe pathological changes.The expression levels of inflammation-related factors were detected by real time quantitative PCR(qPCR),while the levels of malondialdehyde(MDA),H2O2,and 8-hydroxyguanine(8-OHG)were detected via biochemical experiments.Additionally,RNA damage was assessed by reverse transcription blocking combining with double primer PCR.Results GPx3-modified MSCs significantly improved the pathological damage in post-radiation lung tissues and inhibited the fibrosis process and inflammatory response.GPx3-modified MSCs were able to scavenge reactive oxygen species(ROS)more effectively,resulting in a reduction of lipid peroxidation products such as MDA and oxidative damage to RNA formation of 8-OHG.Conclusion By decreasing ROS accumulation,GPx3-modified MSCs can potentially reduce oxidative damage and attenuate RILI.GPx3-modified MSCs can improve the therapeutic efficacy against RILI.
