Advances in CRISPR-Cas12a in detection of foodborne pathogenic bacteria
10.7644/j.issn.1674-9960.2024.06.011
- VernacularTitle:CRISPR-Cas12a在食源性致病菌检测中的应用研究进展
- Author:
Zishu LAN
1
;
Tianjiao CHEN
;
Junwen LI
;
Min JIN
;
Dong YANG
Author Information
1. 军事科学院军事医学研究院,天津 300050
- Keywords:
CRISPR-Cas12a;
foodborne pathogenic bacteria;
water and food security;
on-site testing
- From:
Military Medical Sciences
2024;48(6):474-480
- CountryChina
- Language:Chinese
-
Abstract:
Foodborne pathogens make much difference to food safety,and troops are also at risk of infection during military operations and daily lives,so there is an urgent need for highly sensitive and rapid detection technologies.In recent years,with the continuous development of clustered regularly interspaced short palindromic repeats(CRISPR)technology,the CRISPR-Cas system with trans-cutting activity has received increasing attention.This paper outlines the basic principles of CRISPR-Cas12a-based detection technology and conventional signal readout technologies.It also analyzes the current status and developmentsof CRISPR-Cas12a in detecting foodborne pathogenic bacteria.The challenges to and prospects of this technology are also described.
