Endogenous metabolite nicotinamide alleviates spontaneous lupus nephritis progression in a mouse model
10.7644/j.issn.1674-9960.2024.06.010
- VernacularTitle:内源代谢物烟酰胺缓解小鼠自发性狼疮肾炎的症状
- Author:
Yanyan SHEN
1
;
Yanhong LIU
;
Qiuying HAN
;
Ailing LI
;
Qing XIA
;
Xuemin ZHANG
;
Weina ZHANG
Author Information
1. 国家生物医学分析中心,北京 100039
- Keywords:
systemic lupus erythematosus(SLE);
NZB/W(F1)mice;
nicotinamide;
animal model
- From:
Military Medical Sciences
2024;48(6):468-473
- CountryChina
- Language:Chinese
-
Abstract:
Objective To construct an NZB/W(F1)mouse model of systemic lupus erythematosus and evaluate the effects of nicotinamide on each index of lupus nephritis pathogenesis of NZB/W(F1)mice in order to provide data for research on the role of nicotinamide in the treatment of lupus nephritis.Methods Female NZB/W(F1)mice were obtained by crossing male NZW mice with female NZB ones.Urine samples were collected using metabolic cages and proteinuria test strips were used to detect proteinuria.Blood samples were collected through the orbital venous plexus in mice.Enzyme-linked immunosorbent assay(ELISA)was used to detect the level of anti-dsDNA antibody.Levels of serum creatinine and urea nitrogen and liver function indexes were detected using an automatic blood analyzer.Hematoxylin-eosin staining and immunofluorescence technique were used to detect the pathological state of the kidney.Results The levels of proteinuria,double-stranded DNA antibodies,serum creatinine,and urea nitrogen were gradually increased during the natural course of the disease in female NZB/W(F1)mice,indicating that the lupus nephritis disease model was constructed in female NZB/W(F1)mice.Compared to the control group,nicotinamide feeding could obviously decrease the level of proteinuria(P=0.0070),inhibit the production of double-stranded DNA antibodies(P=0.0325),and retard the progression of serum creatinine(P=0.0067)and urea nitrogen indexes(P=0.0166)in serum.In addition,the pathological state of the kidney in the nicotinamide feeding group was significantly alleviated compared with the control group.Conclusion A lupus nephritis disease model is constructed in NZB/W(F1)mice.Nicotinamide feeding can obviously alleviate the disease state of lupus nephritis in NZB/W(F1)mice.
