A rapid detection method for multiple serotypes of Salmonella based on multiplex nanopore sequencing
10.7644/j.issn.1674-9960.2024.05.011
- VernacularTitle:一种基于多路纳米孔测序技术的多种血清型沙门菌快速分型方法
- Author:
Dongshuai WANG
1
;
Danyang SHI
;
Junwen LI
;
Min JIN
Author Information
1. 军事科学院军事医学研究院,天津市环境与食品安全风险监督技术重点实验室,天津 300050
- Keywords:
multiplex nanopore sequencing;
Salmonella;
serotypes;
rapid detection
- From:
Military Medical Sciences
2024;48(5):389-394
- CountryChina
- Language:Chinese
-
Abstract:
Objective To develop a localized Salmonella serotyping method based on multiplex nanopore sequencing technology,and evaluate the impact of serotype quantity and sequencing time on the serotyping results,aiming to provide a method for simultaneous rapid serotyping of various Salmonella serotypes.Methods Initially,a multiplex sequencing method was established by using nanopore sequencing technology.Subsequently,a localized Salmonella serotyping workflow was developed based on the coregenome MLST database of Salmonella.Finally,the effects of serotype quantity and sequencing time on serotyping results were evaluated.Results The established method for serotyping various Salmonella serotypes achieved an accuracy and specificity of 100％,with the ability to accurately serotyping at least 6 Salmonella serotypes within 2 hours.Conclusion The multiplex nanopore sequencing method enables rapid serotyping of multiple Salmonella serotypes simultaneously,significantly improving the identification efficiency of Salmonella serotypes and providing robust support for the rapid serotyping of Salmonella serotypes in environmental water samples.
