Properties and antibacterial activity of novel self-assembled antibacterial peptide CR-16
10.7644/j.issn.1674-9960.2024.05.008
- VernacularTitle:新型自组装抗菌肽CR-16的性质及抑菌活性研究
- Author:
Yanan CUI
1
;
Pengfei ZOU
;
Wei GONG
;
Yuli WANG
;
Chunsheng GAO
;
Yang YANG
;
Meiyan YANG
Author Information
1. 军事科学院军事医学研究院,北京 100850
- Keywords:
antimicrobial peptides;
self-assembly;
antibiotic resistance;
Escherichia coli;
antimicrobial activity
- From:
Military Medical Sciences
2024;48(5):369-375
- CountryChina
- Language:Chinese
-
Abstract:
Objective To study the properties and antimicrobial activity of the novel self-assembled antimicrobial peptide(AMP)CR-16,and to provide experimental evidence for the treatment of bacterial infections.Methods CR-16 was designed and synthesized based on the structure of antimicrobial peptides Buforin Ⅱ and LfcinB.Dynamic light scattering(DLS),transmission electron microscopy(TEM),and X-ray diffraction(XRD)were used to characterize CR-16.Based on the results of critical micelle concentration(CMC),the self-assembled properties of CR-16 were investigated using atomic force microscopy(AFM)and circular dichroism(CD).The minimum inhibitory concentration(MIC)was used to study the inhibitory effect of CR-16 while transmission electron microscopy(TEM)was adopted to observe the interactions between CR-16 and the outer membrane of bacteria.Results AMP CR-16 was prepared as self-assemblies,which were regularly spherical in shape and stable in activity.CR-16 could inhibit both the growth of Escherichia coli and,more importantly,the growth of NDM-1-producing carbapenem-resistant Escherichia coli,promising good prospects in treating infections caused by antibiotic-resistant bacteria.Conclusion CR-16 can be self-assembled and deliver antibacterial effects against Escherichia coli.
