Protective effect and mechanism of mesenchymal stem cell derived exosomes against pulmonary oxygen toxicity
10.7644/j.issn.1674-9960.2024.04.004
- VernacularTitle:干细胞外泌体对肺型氧中毒的保护作用和机制
- Author:
Sheng XU
1
,
2
,
3
;
Likun CUI
;
Shu WANG
;
Jie CHEN
;
Xiaochen BAO
;
Yue WANG
;
Yunpeng ZHAO
Author Information
1. 海军军医大学转化医学中心干细胞与再生医学研究室,上海 200433
2. 海军军医大学免疫与炎症全国重点实验室,上海 200433
3. 上海市干细胞临床转化研究院,上海 200092
- Keywords:
exosome;
pulmonary oxygen toxicity;
lung injury
- From:
Military Medical Sciences
2024;48(4):267-272
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the role and mechanism of exosomes derived from mesenchymal stem cells in hyperbaric oxygen caused pulmonary oxygen toxicity.Methods Mice were divided into the control group that was exposed to normal air,and hyperbaric oxygen exposure groups treated with phosphate buffer saline(PBS)or exosomes,respectively.PBS and exosome treatment were given one day prior to exposure.Mice were subjected to 0.23 MPa pure oxygen for 8 hours.The lung wet-dry ratio,inflammation,exudation and pathological injury were analyzed,while cell death and antioxidant related molecules were detected.Results Pretreatment with exosome significantly attenuated lung injury caused by hyperbaric oxygen exposure by decreasing the lung wet-dry ratio,inflammation,and cell apoptosis.Conclusion Prophylactic administration of exosomes derived from mesenchymal stem cells can alleviate hyperbaric oxygen induced pulmonary oxygen poisoning by attenuating cell death and inflammation.
