Physical and Biological Activity of Domestic Product of Modified Bovine Lung Surfactant.
- Author:
Chul LEE
1
;
Min Soo PARK
;
Moon Sung PARK
;
Jung Nyeon KIM
;
Jong Wook LEE
;
Kwang Hyun YOU
;
Won Jae KWAG
;
Kook In PARK
;
Ran NAMGUNG
;
Dong Gwan HAN
Author Information
1. Department of Pediatrics, Yonsei University College of Medicine, Seoul, Korea.
- Publication Type:Original Article
- Keywords:
Modified bovine lung surfactant;
Fetal rabbit lung;
Pressure-lung volume curve;
Surface tension-surface area diagram
- MeSH:
Animal Experimentation;
Awards and Prizes;
Fetus;
Financing, Organized;
Lung*;
Microbubbles;
Pathology;
Respiratory Distress Syndrome, Newborn;
Surface Tension;
Surface-Active Agents
- From:Journal of the Korean Pediatric Society
1997;40(6):771-785
- CountryRepublic of Korea
- Language:Korean
-
Abstract:
PURPOSE: Neonatal respiratory distress syndrome is caused by the deficiency of lung surfactant in premature babies. For the treatment of RDS at present surfactants such as Surfacten (Tokyo-Tanabe Co., Japan) and Exosurf (Wellcome Co., USA) are used. As awarded the grant from the Ministry of Science and Technology for a model research project of Medium-Technology program, we have modified (supplemented) the bovine lung extracts to get YY-38, for which we have performed physical and biological activities. METHODS: For physical properties, we performed stable microbubble test (SMR) and measured surface tension lowering activity using a pulsating bubble surfactometer. Minimum and maximum surface tensions measured at 1 and 5 minutes gave surface tension-surface area diagrams, from which compressibility at surface tension 10mN/m was also calculated. As to the biological activity, we used premature rabbit fetuses as a model for the study of pressure-lung volume relationship. The lung pathology was examined on the lung tissues subsequently obtained, and aerated area ratios were calculated based on the area measured by an image analyzer. RESULTS: The minimum surface tensions of YY-38 at 1 and 5 minutes for all different concentrations were low at 10mN/m, while the maximum surface tensions ranged from 33.01mN/m to 41.07mN/m. The surface tension-surface area curve showed a definite hysteresis at 1 and 5 minutes for all concentrations, and the surface tension fell below 10mN/m with 20% surface area compression. The compressibilities at surface tension 10mN/m at 5minutes for all concentrations were all below 0.02. In animal experiments, the mean lung volume of premature rabbit fetuses was inflated to 80.9ml/kg at maximum 30cmH2O, while the lung volume was maintained at 38.3mg/kg when the lung was deflated to 5cmH2O. The overall aerated area ratio was 45.4%. CONCLUSIONS: YY-38 formed sufficient amount of stable microbubbles and had a surface tension low enough to maintain alveolar stability and to exhibit a good hysteresis curve. In animal experiments it helped the expansion of premature lungs during inspiratory phase and was effective in the prevention of collapse during expiratory phase.
