The Effect of Protein Kinase C Pretreatment on Gliotoxin Induced Apoptosis in H9c2 Cells.
10.11637/kjpa.2000.13.1.119
- Author:
Jung Mu HER
1
;
Jay Min OH
;
Rae Kil PARK
;
Hong Seob SO
;
Yeun Ja MUN
;
Min Kyu CHOI
;
Gab Sang LEE
;
Yeun Tai CHUNG
;
Ock Kyu PARK
Author Information
1. Department of Anatomy, Wonkwang University, Korea.
- Publication Type:Original Article
- Keywords:
Gliotoxin;
apoptosis;
Caspase-3;
Protein kinase C (PKC)
- MeSH:
Apoptosis*;
Aspergillus;
Calcium;
Caspase 3;
Cell Death;
DNA Fragmentation;
Fungi;
Gliotoxin*;
Hydrogen Peroxide;
Protein Kinase C*;
Protein Kinases*
- From:Korean Journal of Physical Anthropology
2000;13(1):119-128
- CountryRepublic of Korea
- Language:Korean
-
Abstract:
Aspergillus funigatus and other pathogenic fungi synthesize a toxic epidithi- odiopiperzine (ETP) metabolite called gliotoxin. Gliotoxin is an epidithiodiopiperzine compound which can both react with sulfhydryl groups and form hydrogen peroxide. The fungal toxin gliotoxin induces apoptotic cell death in a variety of cells. Apoptosis induced by gliotoxin need calcium but effect of calcium preconditioning is unknown by gliotoxin. We studied the effect of protein kinase C and calcium preconditioning on gliotoxin-induced apoptosis in H9c2 cell. PKC and calcium preconditiong inhibited DNA fragmentation by gliotoxin. From this above results suggest that gliotoxin induce apoptosis via caspase-3 activation, because caspase-3 inhibitor (DEVD-CHO) didn't induce apoptosis in gliotoxin treated H9c2 clls. Calcium and PKC preconditioning inhibit caspase-3 activation by gliotoxin. These data means that PKC preconditioning is related with caspase-3 regulate in gliotoxin-induced apoptosis.
