Effects of leptin on hypothalamic neuronal activity and adipose tissue metabolism in obese mice
10.16098/j.issn.0529-1356.2024.04.011
- VernacularTitle:瘦素对肥胖小鼠下丘脑神经元活性及脂肪组织代谢的影响
- Author:
Xiao-Xuan LIU
1
,
2
;
Han-Si ZHANG
;
Xiao-Jing HAN
;
Xiao-Di SHANG
;
Jing KANG
;
Jun-Tang LIN
;
Xin YAN
;
Liang QIAO
Author Information
1. 河南省干细胞与生物治疗工程研究中心,新乡医学院生命科学技术学院,河南新乡 453003
2. 河南省医用组织再生重点实验室,河南新乡 453003
- Keywords:
Obesity;
Leptin;
Hypothalamus;
Adipose tissue;
Immunofluorescence;
Real-time PCR;
Leptin-deficient obese mouse
- From:
Acta Anatomica Sinica
2024;55(4):452-459
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the effects of leptin on hypothalamic neuron activity and lipid metabolism in adipose tissue of obese mice.Methods 10 leptin-deficient obese(ob/ob)mice with homozygous mutation of leptin gene and 10 wild-type(WT)mice born in the same litter were randomly divided into control group and leptin treatment group.The activity of pro-opiomelanocortin(POMC)neurons and tyrosine hydroxylase(TH+)neurons,the morphological changes of adipose tissue and the expression of lipid-related genes were analyzed by immunofluorescent staining,HE staining and Real-time PCR.Results Compared with the WT mice,the ob/ob mice showed decreased activity of POMC neurons and TH+neurons and larger cell diameter in adipose tissue and liver tissue.In addition,the expressions of heat-related genes uncoupling protein 1(UCP1),cytochrome c oxidase subunit 8B(Cox8b)and cell death-inducing DNA fragmentation factor,alpha subunit-like effector A(Cidea)in subcutaneous white fat in ob/ob mice decreased significantly,and the expressions of lipid synthesis-related genes sterol regulatory element binding transcription factor 1(Srebp1)and fatty acid synthase(Fas)increased significantly.After treated with leptin,the activities of POMC and TH+neurons were increased,and the cellular diameter and the degree of vacuolar degeneration were reduced in the adipose tissue and liver.Further analyses showed that the expressions of thermogenesis-related genes and lipolysis-related genes were increased,but expressions of lipid synthesis-related genes were reduced in brown adipose tissue.Conclusion Leptin treatment could prevent the increasing of obesity in ob/ob mice,which is associated with increased lipolysis and reduced lipid synthesis through activation of hypothalamic POMC neurons and peripheral adipose tissue sympathetic nervous system.
