Characteristics and clinical significance of changes in peripheral blood B lymphocyte subsets in patients with chronic hepatitis B
10.11855/j.issn.0577-7402.0566.2024.0108
- VernacularTitle:慢性乙型肝炎患者外周血B淋巴细胞亚群的变化特征及临床意义
- Author:
Hai-Yan WANG
1
,
2
;
Chun-Mei BAO
;
Zhi-Qian FENG
;
Jing WANG
;
Ya-Qun LI
;
Jing LI
;
Hong-Min WANG
;
Li-Li TANG
;
Tao YANG
;
Ruo-Nan XU
;
Fu-Sheng WANG
Author Information
1. 中国科学技术大学附属第一医院,安徽合肥 230001
2. 解放军总医院第五医学中心感染病医学部,国家感染性疾病临床医学研究中心,北京 100039
- Keywords:
hepatitis B virus;
hepatitis B,chronic;
B-lymphocytes;
immunity,humoral;
peripheral blood
- From:
Medical Journal of Chinese People's Liberation Army
2024;49(5):511-518
- CountryChina
- Language:Chinese
-
Abstract:
Objective To analyze the changes of B lymphocyte(B cells)subsets in peripheral blood of patients with chronic hepatitis B(CHB)and to explore its clinical significance.Methods Peripheral blood samples were collected from 37 treatment-na?ve CHB patients who were admitted to the Fifth Medical Center of PLA General Hospital from July 2022 to October 2022,and peripheral blood samples collected from 18 healthy individuals who have received the hepatitis B vaccine as healthy controls(HC).The study subjects'clinical indexes such as age,HBV DNA viral load,HBsAg quantification,HBeAg semi quantification,ALT,AST,and AST/ALT ratio were collected.The change characteristics of the frequency,phenotypic and functional markers of peripheral blood B lymphocytes and their subsets were compared between CHB and HC.Using multi-color flow cytometry,and the correlation between them and clinical indexes was analyzed.Results Frequency analysis of each subset of B cells showed that compared with HC,the frequency of total B cells,transitional B cells and naive B cells was decreased(P<0.05),while the frequency of mature B cells,memory B cells,atypical memory B cells and activated memory B cells was increased in CHB patients(P<0.01).And there was no significant difference in the frequency of resting memory B cells between the two groups(P>0.05).The results of functional analysis showed that compared with HC,the expression levels of CD79b on total B cells,mature B cells,memory B cells,naive B cells,activated memory B cells,atypical memory B cells and resting memory B cells in CHB patients were increased(P<0.05).The expression level of programmed cell death protein-1(PD-1)on atypical memory B cells in CHB patients was also higher than that in HC group(P<0.05).The results of correlation analysis showed that the frequency of total B cells in CHB patients was slightly negatively correlated with age(r=-0.39,P<0.05),while the expression of programmed death-1(PD-1)on total B cells,mature B cells,transitional B cells,memory B cells and naive B cells were slightly positively correlated with age(r>0.36,P<0.05).Conclusions Chronic HBV infection leads to depletion of the frequency and function of a portion of B cells in the peripheral blood of CHB patients,and age is a potential risk factor for the decline in humoral immune function in CHB patients.
