circNHSL1 inhibits proliferation and promotes apoptosis of human breast cancer cell line through regulating miR-125b-5p/HMGB3 axis
10.16352/j.issn.1001-6325.2024.12.1678
- VernacularTitle:circNHSL1通过调控miR-125b-5p/HMGB3轴抑制人乳腺癌细胞系增殖及促凋亡
- Author:
Huan LI
1
;
Xianxin XIE
Author Information
1. 辽宁省肿瘤医院 乳腺内科,辽宁 沈阳 110042
- Keywords:
breast cancer;
circNHSL1;
miR-125b-5p;
high mobility group protein 3(HMGB3)
- From:
Basic & Clinical Medicine
2024;44(12):1678-1684
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the regulation of miR-125b-5p/HMGB3 axis by circNHSL1 on the biological behavior of breast cancer cells.Methods The breast cancer cells T47D were divided into si-NC group,si-circNH-SL1 group,miR-NC group,and miR-125b-5p mimic group and si-circNHSL1+miR-125b-5p inhibitor group.The expression of circNHSL1 and miR-125b-5p in breast cancer tissues and adjacent tissues was determined using RT-qPCR.Western blot was conducted to detect the protein expression of high mobility group protein 3(HMGB3).Dual luciferase reporter gene assay was used to confirm the interaction between circNHSL1 and miR-125b-5p,miR-125b-5p and HMGB3.The proliferation rate was measured by CCK-8 method;the colony formation assay was applied to detect the colony formation numbers;flow cytometry was used to measure cell apoptosis rate;Transwell assay was used to detect the numbers of migratory and invasive cells.Results Compared with adjacent tissues,cir-cNHSL1 and HMGB3 expression in breast cancer tissues was significantly increased(P＜0.05),while the expres-sion of miR-125b-5p was significantly decreased(P＜0.05).miR-125b-5p directly bound to circNHSL1 and HMGB3,respectively.The circNHSL1 knockdown down regulated circNHSL1 and HMGB3 expression and upregu-lated miR-125b-5p expression in T47D cells(P＜0.05).After transfection with miR-125b-5p mimic,miR-125b-5p expression was up-regulated and HMGB3 expression was down-regulated(P＜0.05).Moreover,transfection of miR-125b-5p inhibitor reversed the effect of circNHSL1 silencing on the expression of miR-125b-5p and HMGB3.Low expression of circNHSL1 or high expression of miR-125b-5p increased the cell inhibition rate,colony formation and apoptosis rate of T47D and decreased the number of migratory and invasive cells(P＜0.05).Moreover,trans-fection of miR-125b-5p inhibitor saved the effect of circNHSL1 silencing on cell phenotypes(P＜0.05).Conclusions Interfering with circNHSL1 might promote cell apoptosis and inhibit cell proliferation,migration and invasion in breast cancer cell by up-regulating the miR-125b-5p/HMGB3 axis.
