Berberine attenuates renal injury in rats with chronic renal failure
10.16352/j.issn.1001-6325.2024.12.1663
- VernacularTitle:小檗碱减轻慢性肾衰竭大鼠的肾脏损伤
- Author:
Dandan XIE
1
;
Han LI
;
Baiju WANG
;
Na WANG
;
Hanwen CHEN
;
Lei LIU
Author Information
1. 济宁医学院 临床医学院
- Keywords:
berberine;
chronic renal failure;
fibrosis
- From:
Basic & Clinical Medicine
2024;44(12):1663-1669
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the effect of berberine(BBR)on chronic renal failure(CRF)rats and its mechanism.Methods CRF rat model was established by removing 5/6 kidneys and all rats were randomly divided into sham group,chronic renal failure model group,berberine treatment group and uremic clearance granules(UCG)treatment group with 10 in each.Renal function indexes in each group were examined by an automated bio-chemistry instrument.The level of MMP-2 and MMP-9 were detected by ELISA.The degree of renal fibrosis was observed by PAS and Masson staining microscopy.The expression of NF-κB p65 and IL-6 were detected by immu-nohistochemistry method.Expression of fibrosis-related proteins and TGF-β1/ERK signaling pathway proteins in re-nal tissues was detected by Western blot.Results Compared with sham group,renal function and renal histopatho-logical damage was significantly increased in the model group,and BBR improved renal function and histopathological damage in CRF rats.Compared with the sham group,the serum level of MMP-2 and MMP-9 was significantly de-creased(P＜0.05),the expression of IL-6 and NF-κB p65 was up-regulated(P＜0.05).The expression of FN,α-SMA,Col-Ⅰ,Col-Ⅲ,TGF-β1,and p-ERK1/2 proteins was up-regulated in the model group of rats(P＜0.05),while the BBR treatment significantly reversed the expression of these molecules(P＜0.05).Conclusions BBR may improve inflammation and fibrosis by inhibiting TGF-β1/ERK1/2 pathway,and play a protective role in the kidney of CRF rat.
