Down-regulation of ATM/hnRNPK signaling reduces adriamycin resistance of myeloid leukemia

VernacularTitle:下调ATM/hnRNPK信号降低髓系白血病细胞阿霉素耐药
Author: Jinfang ZHANG ¹ ; Mingyan ZHONG ; Quan YANG ; Bei FENG ; Xingdong LI
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1. 南方医科大学附属广东省人民医院(广东省医学科学院)儿童血液科,广东广州 510080
Keywords: ATM/hnRNPK; autophagy; leukemia; adriamycin; drug resistance
From: Basic & Clinical Medicine 2024;44(12):1638-1643
CountryChina
Language:Chinese
Abstract: Objective To explore the role of ATM/hnRNPK signaling in the adriamycin resistance of acute myeloid leukemia.Methods Expression of ATM was examined in the adriamycin resistant and sensitive leukemia cell strains with Western blot.ATM expression was down-regulated by RNAi and ATM inhibitor in the adriamycin resist-ant cell strains.Expression level of hnRNPK and LC3Ⅰ/Ⅱ was detected by Western blot and adriamycin sensitivity was measured by CCK8 assay before and after modulation of ATM expression.Results ATM was overex-pressed in adriamycin resistant leukemia cell strains.The decreased expression of ATM restored the sensitivity to adriamycin.Expression level of LC3Ⅱ and hnRNPK was consistent with the modulation of ATM expression.Conclusions The ATM/hnRNPK signaling pathway may play a role in the occurrence of adriamycin resistance in acute myeloid leukemia by regulating autophagy.