Galangin alleviates inflammation in rat models with hepatitis B
10.16352/j.issn.1001-6325.2024.11.1551
- VernacularTitle:高良姜素减轻乙型病毒性肝炎模型大鼠的炎性反应
- Author:
Wei WANG
1
;
Baolong MU
;
Wenshuang ZHANG
;
Qinglei WU
;
Huihui ZHANG
;
Zhili CAO
Author Information
1. 河北北方学院附属第二医院 感染疾病科,河北 张家口 075000
- Keywords:
galangin;
adenosine 5'-monophosphate-activated protein kinase/silent information regulator 2 homologue 1 pathway;
Hepatitis B;
liver injury;
inflammation
- From:
Basic & Clinical Medicine
2024;44(11):1551-1556
- CountryChina
- Language:Chinese
-
Abstract:
Objective To explore the effect of galangin(Gal)on inflammation in hepatitis B rats.Methods The rats were randomly divided into control group,hepatitis B group,Gal-L and Gal-H groups,positive drug lamivudine group and Gal-H+AMPK inhibitor(compound C)group with 12 in each.After modeling,medication treatment was performed once a day for 8 weeks.The level of alanine aminotransferase(ALT),total bilirubin(TBIL)and aspartate aminotransferase(AST)in the serum of rats were detected.HE staining microscopy was ap-plied to detect pathological changes in liver tissue.TUNEL staining microscopy was applied to detect cell apoptosis in liver tissue.Chromatin immuno-precipitation was applied to detect HBV viral load in liver tissue.ELISA was ap-plied to detect the levels of monocyte chemotactic protein-1(MCP-1),interleukin-12(IL-12),and tumor necrosis factor-α(TNF-α)in liver tissue.Western blot was applied to detect the expression of caspase-3,Bcl-2 associated X protein(Bax),p-AMPK and SIRT1 proteins in liver tissue.Results Compared with hepatitis B group,the liver damage of rats in the Gal-L group,Gal-H group and lamivudine group was alleviated;The level of serum AST,TBIL,and ALT,apoptosis rate,HBV viral load and the level of MCP-1,IL-12,TNF-α as well as the expression of caspase-3 and Bax proteins in liver tissue were all reduced.The expression of p-AMPK and SIRT1 proteins in liv-er tissue increased(P＜0.05).Compound C baffled inhibitory effects of high-dose Gal on inflammation,cell apopto-sis,and HBV viral load in liver tissue of hepatitis B rats.Conclusions The mechanism of Gal in inhibiting inflam-mation,cell apoptosis and HBV virus replication in hepatitis B rats is potentially attributed to up-regulation of the AMPK/SIRT1 pathway.
