Expression of m6 A-modified methyltransferase-like 14 in the hippocampus of depression-like mice
10.16352/j.issn.1001-6325.2024.10.1376
- VernacularTitle:m6A甲基转移酶样蛋白14在抑郁样小鼠海马中的表达
- Author:
Lin YANG
1
;
Zhi QU
;
Yuan WANG
;
Wenxue YI
;
Jin XIN
;
Xiaonuan YIN
;
Dongxiao DUAN
Author Information
1. 郑州大学 基础医学院 生理学和神经生物学系,河南 郑州 450051
- Keywords:
N6-methyladenosine;
methyltransferase-like protein 14(METTL14);
depression disorder;
RNA modification
- From:
Basic & Clinical Medicine
2024;44(10):1376-1382
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the expression of m6 A methyltransferase-like protein 14(METTL14)in the hippocampus of depression-like mice,and to provide a theoretical basis for further study of the molecular mecha-nism of depression and new drug targets.Methods 1)Twenty-four male C57BL/6J mice were divided into con-trol group and depression model group.The control group was fed normally,and the model group was kept in single animal cage for 4 months and added with Chronic Unpredictable Animal Stress(CUMS)to establish the depression-like model.2)After modeling,depression-like behaviors in model group were tested using forced swimming test(FST),sucrose preference test(SPT)and tail suspension test(TST).3)Twenty-four hours after completion of behavioral test,the hippocampus tissues of mice were collected,and the expression of m6 A methyltransferase-like protein METTL14 in the hippocampus of mice was detected by molecular biology experiments.Results 1)The mice in the model group showed significant depression-like behavior;2)The expression of MET-TL14 in the hippocampus of the model group showed an increased mRNA expression with the increased m6 A modifi-cation.3)In the model group,the expression of METTL14 in hippocampal CA1,CA3 and DG districts increased and there was neuronal damage found.Conclusions m6 A methylation function has been proved to be more active in the hippocampus of depressed mice.
