Fluid shear promotes apoptosis of glomerular endothelial cells by activation of Piezo 1
10.16352/j.issn.1001-6325.2024.09.1236
- VernacularTitle:流体剪切力通过激活Piezo 1蛋白促进肾小球内皮细胞凋亡
- Author:
Menglin LUO
1
,
2
,
3
;
Feng ZHENG
;
Xinyao JI
;
Caihong QI
;
Yuheng XIAO
;
Changchun NIU
Author Information
1. 西南医科大学附属医院 检验科,四川 泸州 646099
2. 重庆市人民医院 检验科,重庆 401121
3. 青海省人民医院检验科,青海 西宁 810007
- Keywords:
fluid shear;
Piezo 1;
glomerular epithelial cell;
apoptosis;
Yoda 1
- From:
Basic & Clinical Medicine
2024;44(9):1236-1242
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the effect of fluid shear(FS)on apoptosis of glomerular epithelial cells(GECs)and the role of Piezo 1 protein in it.Methods GECs(glomerular epithelial cells)of SD rat were cul-tured.Fluid shear stimulation was simulated by a Flexcell-T5000 tensiometer.Apoptosis level was detected by flow cytometry.The expression of Piezo 1 proteins in GECs was detected by immunofluorescence staining.The activating of Piezo 1 channels by fluid shear was observed using Ca2+indicator(Cal-590 AM).The effect of Piezo 1 on apop-tosis in GECs was analyzed after modulating the function or expression of Piezo 1 protein using the chemical activa-tor Yoda1,the inhibitor GsMtx 4 was regulated by lentivirus Lv-shPiezo 1.Results Compared with the blank controlgroup,apoptosis increased in the fluid shear group(P＜0.05).The rate of apoptosis increased with the enhancing of fluid shear strength;Piezo 1 was commonly expressed in GECs.Fluid shear activated Piezo 1 chan-nel and enhanced expression of Piezo 1.The agonist Yoda1 promoted the apoptosis of GECs GsMtx 4 inhibited the apoptosis induced by fluid shear.Lv-shPiezo 1 knocked down the expression of Piezo 1 in GECs and the apoptosis rate of GECs in the knockdown group was reduced as compared to that in the control group and Lv-Ctrl group(P＜0.05).Conclusions Fluid shear may promote apoptosis of GECs by activation of Piezo 1 and by enhancing expression of Piezo 1.
