Culture and characterization of breast cancer organoids

VernacularTitle:乳腺癌类器官的培养及鉴定
Author: Yujia XIA ¹ ; Zhenli YANG ; Di DAI ; Yuqin LIU
Author Information

1. 中国医学科学院基础医学研究所北京协和医学院基础学院病理学系,北京 100005
Keywords: breast cancer; organoid; drug sensitive test
From: Basic & Clinical Medicine 2024;44(9):1223-1228
CountryChina
Language:Chinese
Abstract: Objective To establish breast cancer organoids for a long time and to characterize their molecular ex-pression and test their sensitivity to chemotherapy drugs.Methods Breast cancer specimens were digested with col-lagenase to release cancer cells for organoid culture.The organoids were characterized by morphology and ER,PR,HER-2,CK expression by technology of histoimmunofluorescence.Among them,three were tested for paclitaxel,doxorubicin and cisplatin sensitivity.Results A total of 44 cases of breast cancer organoids were established,all of which showed dense spherical-like growth and ER,PR,HER-2,CK,matching their clinical counterpart.Breast cancer organoids were sensitive to chemotherapy drugs paclitaxel,doxorubicin and cisplatin.Conclusions Organoid model,as a new in vitro may reproduce the pathophysiology features with heterogeneity.