Knockdown of lncRNA UCA1 reduces gemcitabine resistance of human bladder cancer cell line T24
10.16352/j.issn.1001-6325.2024.08.1113
- VernacularTitle:敲降lncRNA UCA1降低人膀胱癌细胞系T24对吉西他滨耐药
- Author:
Changdong ZHOU
1
;
Yang LIN
;
Kai SUN
;
Yuxin TIAN
Author Information
1. 吉林省肿瘤医院泌尿外科,长春 130012
- Keywords:
bladder cancer;
lncRNA UCA1;
Bcl-2;
autophagy;
gemcitabine
- From:
Basic & Clinical Medicine
2024;44(8):1113-1119
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the in vitro effect of lncRNA UCA1 on gemcitabine(GEM)resistance of blad-der cancer cell line T24 and its related molecular mechanism.Methods The mRNA expression of UCA1 in T24 cells and in T24/GEM cells was detected by RT-qPCR.The T24/GEM cells were incubated with varying concentra-tions(0.1,1,and 10 μmol/L)of GEM for 48 hrs.LC3 staining microscopy was employed to visualize autophagic puncta,while the expression of autophagy-related proteins was assessed by Western blot.UCA1-shRNA and UCA1-shRNA+pcDNA-Bcl-2 were transferred into T24/GEM cells,the sensitivity of cells to GEM was evaluated by MTT method and flow cytometry;the expressions of p53 and Bcl-2 were detected by Western blot.Results The expres-sion level of UCA1 in T24/GEM cells was significantly higher than that of parental T24 cells(P＜0.05).The con-centration of GEM in the range of 0-10 p.mol/L significantly induced dose-dependent autophagy in T24/GEM cells(P＜0.05).Knockdown of UCA1 enhanced the sensitivity of T24/GEM cells to GEM(P＜0.05),while reducing autophagy(P＜0.05)and down-regulating the expression of p53 and Bcl-2(P＜0.05).Over-expression of Bcl-2 partially reversed the GEM sensitization and autophagy inhibition of UCA1-shRNA in T24/GEM cells(P＜0.05).Conclusions Knockdown of lncRNA UCA1 reduces GEM resistance of T24/GEM cells by inhibiting Bcl-2 mediated autophagy.
