A novel M2e-multiple antigenic peptide providing heterologous protection in mice.
- Author:
Feng WEN
1
;
Ji Hong MA
;
Hai YU
;
Fu Ru YANG
;
Meng HUANG
;
Yan Jun ZHOU
;
Ze Jun LI
;
Xiu Hui WANG
;
Guo Xin LI
;
Yi Feng JIANG
;
Wu TONG
;
Guang Zhi TONG
Author Information
- Publication Type:Original Article ; Research Support, Non-U.S. Gov't
- Keywords: H3N2; M2e-multiple antigenic peptide; high-yield; inactivated vaccine; swine influenza virus
- MeSH: Animals; Antibodies, Viral/blood; Antigens, Viral/genetics/*immunology; Body Weight; Cross Protection/*immunology; Disease Models, Animal; Epitopes, T-Lymphocyte/genetics/immunology; Female; Influenza A Virus, H3N2 Subtype/genetics/*immunology; Influenza Vaccines/*immunology; Mice; Mice, Inbred BALB C; Orthomyxoviridae Infections/*immunology/mortality/pathology/prevention & control; Peptides/genetics/*immunology; Random Allocation; Survival Analysis; Vaccines, Synthetic/immunology; Virus Replication
- From:Journal of Veterinary Science 2016;17(1):71-78
- CountryRepublic of Korea
- Language:English
- Abstract: Swine influenza viruses (SwIVs) cause considerable morbidity and mortality in domestic pigs, resulting in a significant economic burden. Moreover, pigs have been considered to be a possible mixing vessel in which novel strains loom. Here, we developed and evaluated a novel M2e-multiple antigenic peptide (M2e-MAP) as a supplemental antigen for inactivated H3N2 vaccine to provide cross-protection against two main subtypes of SwIVs, H1N1 and H3N2. The novel tetra-branched MAP was constructed by fusing four copies of M2e to one copy of foreign T helper cell epitopes. A high-yield reassortant H3N2 virus was generated by plasmid based reverse genetics. The efficacy of the novel H3N2 inactivated vaccines with or without M2e-MAP supplementation was evaluated in a mouse model. M2e-MAP conjugated vaccine induced strong antibody responses in mice. Complete protection against the heterologous swine H1N1 virus was observed in mice vaccinated with M2e-MAP combined vaccine. Moreover, this novel peptide confers protection against lethal challenge of A/Puerto Rico/8/34 (H1N1). Taken together, our results suggest the combined immunization of reassortant inactivated H3N2 vaccine and the novel M2e-MAP provided cross-protection against swine and human viruses and may serve as a promising approach for influenza vaccine development.
