Repair effect of human amniotic mesenchymal stem cells on uterine scars in rats
10.16352/j.issn.1001-6325.2024.07.1002
- VernacularTitle:人羊膜间充质干细胞对大鼠子宫瘢痕的修复作用
- Author:
Jia SONG
1
;
Feng ZHAO
;
Ting ZHANG
;
Jing XU
;
Jingli SUN
;
Zhenyu CHEN
Author Information
1. 北部战区总医院 妇产科,辽宁 沈阳 110016
- Keywords:
human amniotic mesenchymal stem cell;
cesarean section;
uterine scar
- From:
Basic & Clinical Medicine
2024;44(7):1002-1007
- CountryChina
- Language:Chinese
-
Abstract:
Objective To exploring the effect of human amniotic mesenchymal stem cells(hAMSCs)on the re-pair of rat uterine scars.Methods The hAMSCs were isolated and cultured,Female SPF grade SD rats were selected for full-thickness incision of uterine wall and then implanted with hAMSCs.On the 30th day after op-eration,the uterine incision was examined histologically.ImageJ image analysis software was used to analyze and to compare the thickness of uterine myometrium and the percentage of fibrotic area in each group.Immuno-histochemical method was used for detecting the percentage of positive areas of α-SMA,TGF-β1,and Ki-67.Results Compared with the PBS group,the hAMSCs group showed significant thickening of the uterine mus-cle layer and fibrotic area was decreased,The positive expression of α-SMA,and Ki-67 significantly increased(P＜0.05),while the expression of TGF-β1 was significantly reduced(P＜0.05).Conclusions The hAMSCs may promote the repair of uterine incision scars by reducing the formation of scar fibrosis and promoting the proliferation of smooth muscle cells in uterine scars.
