Recombinant human lactoferrin accelerates fracture healing in rats by promoting osteoblast proliferation
10.16352/j.issn.1001-6325.2024.07.0989
- VernacularTitle:重组人乳铁蛋白通过促进成骨细胞增殖加速大鼠骨折愈合
- Author:
Junwu YE
1
,
2
;
Xuzhou ZHENG
;
Lincong FEI
;
Shuyang LI
;
Tianfu YANG
Author Information
1. 西南医科大学附属医院 骨与关节外科,四川 泸州 646000
2. 四川省骨科置入器械研发及应用技术工程实验室,四川 泸州 646000
- Keywords:
recombinant human lactoferrin;
fracture healing;
osteoblast;
femur fracture;
rat
- From:
Basic & Clinical Medicine
2024;44(7):989-996
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the effect of recombinant human lactoferrin(rhLF)on fracture healing in rat models with femur fracture and the proliferation of rat primary osteoblasts.Methods Male rats were randomly di-vided into 3 groups:simple fracture group(SF group),collagen film-treated group(CF group)and rhLF-com-pound collagen lamination film-treated group(CLF group).The fracture healing was observed by X-ray image and histological microscopy with HE and Masson staining.The newborn SD rats with osteoblasts were treated with differ-ent concentrations of rhLF,and osteoblast proliferation was detected by MTT method.Results The bone scabs in the CLF group were significantly larger than those in the SF and CF groups at 1,2,and 3 weeks postoperatively(P＜0.05).There was no difference in the comparison between the groups at 4th week.The proliferation of cartilag-inous and sclerotic bone scabs in the CLF group was more pronounced and showed the appearance of mature trabec-ulae and lamellar bone with a high degree of maturation of the bone scabs.The groups with 100 mg/L and 500 mg/L rhLF showed a significant decrease in A value for rat primary osteoblasts cultured in vitro as compared with the control group(P＜0.05).The A value increased significantly at the first day(P＜0.05).The concentration of rhLF increased at the fifth and tenth days.Conclusions rhLF has an accelerating effect on fracture healing which is manifested by fast scab formation,large volume and high maturity.The rhLF can promote the proliferation of os-teoblasts cultured in vitro,and the effect is mainly manifested in the rapid proliferation stage of osteoblasts.
