Impact of MAFB on polarization and function of tumor associated macrophages
10.16352/j.issn.1001-6325.2024.07.0965
- VernacularTitle:MAFB对肿瘤相关巨噬细胞极化及功能的影响
- Author:
Xiaotian CHEN
1
;
Chong CHEN
;
Yunping LUO
Author Information
1. 中国医学科学院基础医学研究所 北京协和医学院基础学院 免疫学系,北京 100005
- Keywords:
MAFB;
macrophage;
M1/M2 polarization;
phagocytosis;
tumor cytotoxicity
- From:
Basic & Clinical Medicine
2024;44(7):965-973
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the impact of the MAFB transcription factor on macrophage polarization phe-notype and function.Methods Analysis of correlation of MAFB expression with survival period of patients of breast cancer and lung adenocarcinoma was performed by RNA-seq data from the Kaplan-Meier database.Flow cytometry was applied to detect MAFB expression in primary human monocytes.Expression of MAFB mRNA or protein of hu-man acute monocytic leukaemia cell line(THP-1)was detected by RT-qPCR or Western blot.Detection of macro-phages phenotypes was performed by macrophages polarization in vitro,co-culture,RT-qPCR and flow cytometry.Phagocytosis assay was used to assess changes in phagocytic ability of macrophages.In vitro killing assay to evaluate changes in tumor-killing ability of macrophages.Results Compared to the MAFB low-expression group,patients with high MAFB expression in breast cancer and lung adenocarcinoma exhibited a decreased survival period(P＜0.05).Protein interaction networks showed a correlation between MAFB protein and macrophage polarization proteins.Compared to other cell type,MAFB was specifically highly expressed in human monocyte-macrophage cells.Phorbol myristate ester(PMA)induced an increased MAFB expression in THP-1 cells(P＜0.05).Knockdown of MAFB inhibited the expression of M2-related genes(P＜0.05).Co-culture with tumor cells and knockdown of MAFB suppressed expression of polarization gene(P＜0.05).Knockdown of MAFB did not affect the phagocytic ability P＞0.05)but enhanced tumor-killing ability of macrophages(P＜0.05).Conclusions This investigation proves potential function of MAFB transcription factor in regulation of macrophage M2 polarization and anti-tumor effect in solid tumors.
