Expression of neurotrophic factors in injured spinal cord after transplantation of human-umbilical cord blood stem cells in rats.
- Author:
Hyo Jin CHUNG
1
;
Wook Hun CHUNG
;
Jae Hoon LEE
;
Dai Jung CHUNG
;
Wo Jong YANG
;
A Jin LEE
;
Chi Bong CHOI
;
Hwa Seok CHANG
;
Dae Hyun KIM
;
Hyun Jung SUH
;
Dong Hun LEE
;
Soo Han HWANG
;
Sun Hee DO
;
Hwi Yool KIM
Author Information
- Publication Type:Original Article ; Research Support, Non-U.S. Gov't
- Keywords: brain-derived neurotrophic factor; nerve growth factor; neurotrophic factor; neurotrophin-3; spinal cord injury
- MeSH: Animals; Brain-Derived Neurotrophic Factor/*genetics; *Cord Blood Stem Cell Transplantation; Enzyme-Linked Immunosorbent Assay; Gene Expression Profiling; *Gene Expression Regulation; Humans; Locomotion; Nerve Growth Factor/genetics; Rats; Spinal Cord Injuries/*therapy
- From:Journal of Veterinary Science 2016;17(1):97-102
- CountryRepublic of Korea
- Language:English
- Abstract: We induced percutaneous spinal cord injuries (SCI) using a balloon catheter in 45 rats and transplanted human umbilical cord blood derived mesenchymal stem cells (hUCB-MSCs) at the injury site. Locomotor function was significantly improved in hUCB-MSCs transplanted groups. Quantitative ELISA of extract from entire injured spinal cord showed increased expression of brain-derived neurotrophic factor (BDNF), nerve growth factor (NGF) and neurotrophin-3 (NT-3). Our results show that treatment of SCI with hUCB-MSCs can improve locomotor functions, and suggest that increased levels of BDNF, NGF and NT-3 in the injured spinal cord were the main therapeutic effect.
