Change of cell cycle in rat spleen after severe abdominal infection
10.16352/j.issn.1001-6325.2024.06.0828
- VernacularTitle:大鼠严重腹腔感染后脾脏细胞周期的变化
- Author:
Jinping LI
1
;
Hanqing LIU
;
Quanhui YANG
Author Information
1. 国家癌症中心/国家肿瘤临床医学研究中心/中国医学科学院 北京协和医学院肿瘤医院 重症医学科,北京 100021
- Keywords:
spleen;
severe sepsis;
cell cycle;
apoptosis;
p27
- From:
Basic & Clinical Medicine
2024;44(6):828-832
- CountryChina
- Language:Chinese
-
Abstract:
Objective To explore the mortality rate and changes in splenic cell cycle and apoptosis in rats with ab-dominal infection.Methods An animal model of sepsis was induced by cecal ligation and puncture(CLP)in rats.At serial time points 0 h,6 h,12 h,24 h,48 h,and 72 h after CLP,the animal death rate was calculated.The percentage of cell cycle G1,S,G2/M phase and apoptosis of isolated spleen cells were analyzed using flow cytome-ter.The expression of p27 of spleen tissue was determined by Western blot analysis.Results In the rat model of CLP-induced systemic inflammatory response,the mortality of animals gradually increased at 0 h,6 h,12 h,24 h,48 h and 72 h after CLP surgery,reaching a maximum of 88.81%.The abdominal infection in the animals gradual-ly worsened at 0 h,6 h,12 h,and 24 h after CLP surgery,but localized and gradually alleviated after 48 h and 72 h.Within 48 h after CLP surgery,there was a blockage in the G1 phase of the spleen cell cycle,an increase in the percentage of apoptotic cells,and an elevation in p27 expression.After 48 h,the G1 phase blockage gradually recovered,the number of apoptotic cell decreased,and p27 expression declined.Additionally,with-in 48 h after CLP surgery,there was a decrease in the percentage of cells in the S and G2/M phases of the spleen cell cycle,but the percentage of cells in these phases gradually increased after 48 h.Conclusions Cell cycle arrest and apoptosis of the spleen cells are potentially contributed to the change of animal mortality after abdominal infection.
