Datura metel L.inhibits the secretion of inflammatory factors and angiogenesis in keratinocytes in vitro
10.16352/j.issn.1001-6325.2024.06.0816
- VernacularTitle:洋金花体外抑制角质形成细胞炎性因子分泌和血管形成
- Author:
Boping ZHANG
1
,
2
;
Xinlei SI
;
Fenfang WU
Author Information
1. 北京中医药大学东直门医院,北京 100700
2. 北京中医药大学深圳医院(龙岗) 中心实验室,广东 深圳 518172
- Keywords:
psoriasis;
Datura metel L.;
CCL20;
angiogenesis
- From:
Basic & Clinical Medicine
2024;44(6):816-820
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the potential role of the traditional herbal medicine Datura metel L.in the treatment of psoriasis using TNF-α-induced inflammation in keratinocytes as a model.Methods Keratinocyte cell line HaCaT was used to establish a psoriasis cell model by tumor necrosis factor-alpha(TNF-α)treatment.The ex-periment comprised three groups:a blank control group,TNF-α-induced psoriasis model group,and TNF-α+Datura metel L.intervention group.Level of IL-17 and CCL20 was measured ELISA,expression of nuclear factor kappa B(NF-κB)subunit p65 protein was measured by Western blot.Endothelial cell tube formation experiment was conducted using an in vitro angiogenesis analysis kit.Results Compared to the TNF-α-induced psoriasis model group,the Datura metel L.extract significantly reduced the levels of IL-17 and CCL20 in the cell culture su-pernatant of TNF-α-induced psoriasis model(P＜0.001);Datura metel L.extract markedly decreased the NF-κB subunit p65 protein level in TNF-α-induced psoriasis model cells(P＜0.01);Datura metel L.extract effectively inhibited the induction of endothelial cell tube formation by the cell culture supernatant of the psoria-sis model group.Conclusions The Datura metel L.extract down-regulates NF-κB signaling pathway mole-cules,reducing the production of IL-17 and CCL20 inflammatory factors in inflammatory keratinocytes and in-hibiting angiogenesis.
