Effects of intraperitoneal injection of busulfan on metabolic characteristics of spermatogonial stem cells
10.16352/j.issn.1001-6325.2024.06.0793
- VernacularTitle:腹腔注射白消安对精原干细胞代谢特征的影响
- Author:
Zhixin YU
1
;
Xinyu MANG
;
Dingfeng ZOU
;
Shiying MIAO
;
Wei SONG
;
Kai LI
Author Information
1. 中国医学科学院基础医学研究所 北京协和医学院基础学院 生物化学与分子生物学系重大疾病共性机制研究全国重点实验室,北京 100005
- Keywords:
busulfan;
reproductive toxicity;
metabolomics;
spermatogonial stem cells
- From:
Basic & Clinical Medicine
2024;44(6):793-799
- CountryChina
- Language:Chinese
-
Abstract:
Objective To establish a mouse model treated with busulfan and to investigate its effects on the metabo-lism of spermatogonial stem cells(SSCs)of mouse testis.Methods C57BL/6J male mice with age of 8 weeks were injected with 10 mg/kg of busulfan intraperitoneally,then Thy1 positive cells were selected by immunomagnetic beads on day 0,day 5 and day 10 and followed by identification for purity and metabolomic analysis.Results The testis weight ratio decreased and the tissue structure of testis was damaged(P＜0.05).Based on the results of principal component analysis(PCA)and partial least squares discriminant analysis(PLS-DA),there were signifi-cant metabolic differences between the sample groups treated for 0 d,5 d and 10 d.A total of 89 differential metabolites were identified including glutathione(GSH),arginine and unsaturatedfatty acids(UFAs),and their important metabolic pathways involved glycerophospholipid metabolism,arginine and proline metabolism.Conclu-sions Affecting the specific metabolic pathway may result in obvious reproductive toxicity and lead to decrease of testicular weight as well as tissue structure damage in mice.Metabolomic analysis showed that the potential repro-ductive toxicity mechanism of SSCs may be related to the metabolic pathways such as lipid metabolism,arginine and proline metabolism.
