Research on the effects of simulated microgravity on the proliferation of skin stem cells
10.16289/j.cnki.1002-0837.2024.02005
- VernacularTitle:模拟微重力环境对皮肤干细胞增殖影响的研究
- Author:
Siyuan WANG
1
;
Yingsong ZHAO
;
Yingjun TAN
;
Liujia SHI
;
Xuemin YIN
;
Jianghai CHEN
Author Information
1. 华中科技大学同济医学院附属协和医院,武汉 430014
- Keywords:
simulated microgravity;
skin stem cells;
proliferation;
suspension culture condition
- From:Space Medicine & Medical Engineering
2024;35(2):99-104,111
- CountryChina
- Language:Chinese
-
Abstract:
Objective To study the changes and mechanism of skin stem cells in microgravity.Methods The skin stem cells of SD rats were used to establish a suspension culture system and compare the proliferation of skin stem cells with 1G gravity.Results The simulated microgravity significantly affected the velocity of skin stem cell sphere proliferation in suspension culture,which was about 12%higher than the 1G gravity group.Transcriptome sequencing showed that 1673 genes were up-regulated and 1409 genes were downregulated;Calcium signaling;cytokine-cytokine receptor interaction and PPAR pathway were different in the two environments.Conclusion Simulation of microgravity can affect the proliferation behavior of skin stem cells in suspension culture by regulating the expression of key signaling pathways,which provides an experimental basis for further research in spatial microgravity environ ment.
