Expression of IAP Genes During TPA-Induced Differentiation of HL-60 Cells.
- Author:
Won Ki BAEK
1
;
Taeg Kyu KWON
;
Jong Wook PARK
;
Min Ho SUH
Author Information
1. Department of Microbiology, Keimyung University School of Medicine, Korea. wonki@dsmc.or.kr
- Publication Type:Original Article
- Keywords:
Differentiation;
IAP;
HL-60;
Apoptosis
- MeSH:
Apoptosis;
Caspase 3;
HL-60 Cells*;
Humans;
Leukemia
- From:Journal of Bacteriology and Virology
2003;33(1):113-118
- CountryRepublic of Korea
- Language:Korean
-
Abstract:
HL-60 cells (human promyelocytic leukemia cells) differentiate into the monocyte/macrophage like cells that die spontaneously by apoptosis when treated with 12-O-tetradecanoylphorbol-13-acetate (TPA). It is known that inhibitors of apoptosis proteins (IAP) bind to and inhibit caspase 3, 7, 9 activity and the induction of apoptosis. In this study, we examined the expression of IAP genes during TPA induced differentiation of HL-60 cells. During the differentiation, HIAP-1, HIAP-2, and XIAP expressions were decreased in protein levels. The pan-caspase inhibitor z-VAD-fmk blocked the decrease of HIAP-1 and HIAP-2, which indicates HIAP-1 and HIAP-2 could be caspase substrates. These findings suggest that the decrease of IAP proteins is related to the induction of apoptosis that is associated with TPA- induced HL-60 cell differentiation.
