Effect of tail suspension simulated weightlessness on DNA methylation profiles in liver and colon of mice
10.16289/j.cnki.1002-0837.2024.01.009
- VernacularTitle:尾悬吊模拟失重对小鼠肝脏及结肠DNA甲基化谱的影响
- Author:
Yan LU
1
,
2
;
Mingzhou GUO
;
Huikai LI
;
Qianqian CHEN
;
Xiaofeng QIU
;
Enqiang LINGHU
Author Information
1. 中国人民解放军医学院,北京 100853
2. 中国人民解放军总医院第一医学中心消化内科医学部,北京 100853
- Keywords:
simulated weightlessness;
mouse;
liver;
colon;
whole genome;
methylation site;
methylation region
- From:Space Medicine & Medical Engineering
2024;35(1):47-51
- CountryChina
- Language:Chinese
-
Abstract:
Objective This study uses whole-genome methylation capture sequencing technology to screen differential sites and regions of gene methylation in mouse liver and colon under simulated weightlessness conditions to reveal the specific impact of weightlessness on gene methylation.Methods Six 8-week-old male C57BL/6J mice were randomized into the tail suspension group and the control group,with 3 in each.The 3 mice in the tail suspension group recieved tail suspension for simulated weightlessness for 42 days.After the experiment,DNA was extracted from liver and colon tissue and analyzed using genome-wide methylation capture sequencing technology.Results DNA analysis of liver tissue showed that a total of 7 517 differentially methylated sites and 997 differentially methylated regions were found,involving 4 892 genes.DNA analysis of colon tissue revealed 70 340 differentially methylated sites and 12 004 differentially methylated regions,affecting 12 877 genes.GO and KEGG path analysis revealed that these differentially methylated genes were mainly involved in protein binding,cell adhesion,cell activation,and various metabolic pathways.Conclusion This study successfully identified differential methylation sites and regions in mouse liver and colon under simulated weightlessness conditions through high-throughput sequencing technology.These findings help to further understand the impact of long-term space residence on biological gene methylation.It provides new research ideas for the prevention and early treatment of space flight-related diseases.
