Changes of allergenecity of salted and fermented shrimp.
- Author:
Sang Woon BAE
1
;
Soo Young CHOI
;
Hyun Sun JIN
;
Cheol Woo KIM
;
Kwang Eun LEE
;
Eun Seok KANG
;
Jun Yong PARK
;
Sung Pil HONG
;
Hoon Young CHOI
;
Jae Hun JUNG
;
Yong Soo KIM
;
Chein Soo HONG
Author Information
1. Department of Internal Medicine, Institute of Allergy, Yonsei University College of Medicine, Korea. cshong@yumc.yonsei.ac.kr
- Publication Type:Original Article
- Keywords:
salted and fermented shrimps;
shrimps;
allergenecity
- MeSH:
Allergens;
Complex Mixtures;
Electrophoresis, Polyacrylamide Gel;
Enzyme-Linked Immunosorbent Assay;
Fermentation;
Immunoblotting;
Immunoglobulin E
- From:Journal of Asthma, Allergy and Clinical Immunology
2003;23(1):44-52
- CountryRepublic of Korea
- Language:Korean
-
Abstract:
BACKGROUND AND OBJECTIVE: Shrimp is one of the most common food allergens. The salted and fermented shrimp (S/F shrimp) is a traditional food and ingested frequently in the daily life of many Korean people. But few studies have been investigated on the allergenicity of S/F shrimp. The aim of our study is to observe the allergenicity of S/F shrimp and to compare it with the allergenicity of shrimp. MATERIAL AND METHODS: Crude extracts were made from three kinds of S/F shrimps and four kinds of shrimps after boiling. The extracts were used for SDS-PAGE, ELISA, ELISA inhibition test, immunoblotting, and immunoblotting inhibition test with sera from 10 subjects who have shrimp specific IgE by radioallergosorbent (RAST) (3+ or 4+). RESULTS: The protein concentrations of S/F shrimp extracts were less than half of the extracts of shrimps (1.9 mg/ml, 1.2 mg/ml, 1.1 mg/ml vs 4mg/ml, 3.83 mg/ml, 6.8 mg/ml, 6.6 mg/ml). The extracts of S/F shrimp showed weaker and more diffusely distributed protein bands in SDS-PAGE and showed less than half of the O.D. value in IgE-ELISA compared to those of shrimp extracts. The specific IgE immunoblotting of S/F shrimp extracts showed 9 bands (36, 33, 30, 29, 28, 26, 23, 20, 19 kDa). Dose dependent inhibition was observed between shrimps and S/F shrimp in ELISA-inhibition and immnunoblotting inhibition test. The allergen concentration of S/F shrimp needed to inhibit 50% of shrimp-lgE ELISA was five times higher than that of shrimp. CONCLUSION: The crude extracts of S/F shrimps showed less than half the amount of protein compared to crude extracts of shrimps have and also showed reduced allergenicity by salting and fermentation.
