Effects of Huayu Xiaozheng Granules on Angiogenesis and Invasion in Trophoblast Cells with Overexpression of MiR-210 and Under Hypoxia
10.13359/j.cnki.gzxbtcm.2024.11.026
- VernacularTitle:化瘀消癥颗粒对miR-210过表达与低氧状态下滋养细胞血管生成、侵袭的影响
- Author:
Wen-Jing LU
1
;
Shuo YUAN
;
Jia-Han QIU
;
Pin QIU
;
Gao-Pi DENG
Author Information
1. 广州中医药大学第一临床医学院,广东 广州 510400
- Keywords:
Huayu Xiaozheng Granules;
miR-210 overexpression;
hypoxia;
angiogenesis;
invasion;
trophoblast cells
- From:
Journal of Guangzhou University of Traditional Chinese Medicine
2024;41(11):2992-2998
- CountryChina
- Language:Chinese
-
Abstract:
Objective To observe the effect of Huayu Xiaozheng Granules on angiogenesis and invasion in trophoblast cells with overexpression of miR-210 and under hypoxia.Methods HTR-8/SVneo cells were cultured in vitro,a miR-210 overexpression model was established by transfection,and a chemical hypoxia model was established by CoCl2 method.The blank group,miR-210 overexpression group,miR-210 overexpression-negative group,miR-210 overexpression+Chinese medicine group,hypoxia+Chinese medicine group and Chinese medicine group in experiment were set up.Western Blot method was used to detect protein expressions of hypoxia-inducible factor 1α(HIF-1α),vascular endothelial growth factor(VEGF),and invasion-associated factors[matrix metalloproteinase 9(MMP-9)and tissue inhibitors of matrix metalloproteinase 1(TIMP-1)]in HTR-8/SVneo cells.Results Compared with the blank group,the protein expression levels of HIF-1α,VEGF,MMP-9,TIMP-1 and MMP-9/TIMP-1 ratio were increased in the miR-210 overexpression group(P＜0.05);compared with the miR-210 overexpression group,the HIF-1α,VEGF,MMP-9,TIMP-1 protein expression levels and MMP-9/TIMP-1 ratio in the miR-210 overexpression-negative group,miR-210 overexpression+Chinese medicine group,hypoxia+Chinese medicine group and Chinese medicine group were all decreased(P＜0.05),and the decrease effects in Chinese medicine group was especially obvious;compared with the miR-210 overexpression+Chinese medicine group,the protein expression levels of HIF-1α,VEGF,MMP-9,TIMP-1 and MMP-9/TIMP-1 ratio in hypoxia+Chinese medicine group and Chinese medicine group were all decreased(P＜0.05);compared with the hypoxia+Chinese medicine group,the above indicators were decreased in the Chinese medicine group(P＜0.05).Conclusion Huayu Xiaozheng Granules can negatively regulate the increased angiogenesis and invasion of trophoblast cells with miR-210 overexpression and under hypoxia,which may be one of the action mechanisms of its treatment for tubal pregnancy.
