Study on the Mechanism of Quercetin Intervention in Breast Cancer with Depressive Characteristics Based on Network Pharmacology and Animal Experiments
10.13359/j.cnki.gzxbtcm.2024.10.039
- VernacularTitle:基于网络药理学和动物实验探讨槲皮素干预具抑郁特征乳腺癌的作用机制
- Author:
Ying-Chao WU
1
,
2
,
3
,
4
;
Yu-Qi LIANG
;
Yu-Yu HU
;
Liu-Shan CHEN
;
Peng WU
;
Qian ZUO
;
Qian-Jun CHEN
Author Information
1. 广州中医药大学第二临床医学院,广东广州 510405
2. 广州中医药大学第二附属医院中医证候全国重点实验室,广东广州 510120
3. 广东省中医院乳腺科,广东广州 510120
4. 广东省中医药科学院,广东广州 510120
- Keywords:
quercetin;
breast cancer;
depression;
apoptosis;
network pharmacology;
experimental verification;
mice
- From:
Journal of Guangzhou University of Traditional Chinese Medicine
2024;41(10):2795-2803
- CountryChina
- Language:Chinese
-
Abstract:
Objective To explore the mechanism of quercetin in the treatment of breast cancer with depressive features using network pharmacology and animal experiments.Methods Network pharmacology and bioinformatics methods were used to predict the key targets and mechanisms of quercetin in the treatment of breast cancer with depressive characteristics.The predicted results were verified by animal experiments.A mouse model of breast cancer with depressive characteristics was constructed,and quercetin intervention was performed after grouping.The depression of mice was evaluated by open field test.The tumor volume and tumor mass were measured.The expression of Ki-67 in tumor tissue was detected by immunohistochemical staining.The expressions of tumor necrosis factor α(TNF-α),interleukin 6(IL-6),p53,Caspase-3 and B-cell lymphoma/leukemia 2(Bcl-2)in tumor tissue were detected by Western Blot.Terminal deoxynucleotidyl transferase-mediated dUTP nick end labeling(TUNEL)method was used to detect the apoptosis of tumor cells.Results In the breast cancer model group with depressive characteristics,the total movement distance in the open field test and the ratio of residence time in the central area of the open field test were decreased,the tumor volume and tumor mass were significantly increased,and Ki-67 expression level in the tumor tissue was significantly increased,the expression levels of TNF-α,IL-6,p53 and Caspase-3 in the tumor tissue were decreased and the expression level of Bcl-2 was increased,as well as the rate of TUNEL positive cells was decreased,the differences being statistically significant compared with the control group(P＜0.01 or P＜0.001).Compared with the model group,the above indexes were significantly reversed in the quercetin group(P＜0.01 or P＜0.001).Conclusion Quercetin can effectively inhibit the progression of breast cancer with depressive characteristics in mice,and its mechanism is related to the regulation of TNF,IL6,TP53,CASP3,BCL2 and other targets to promote tumor cell apoptosis.
