Study on the Characteristics of Gut Flora Related to Dampness Syndrome in Population at Risk of Cerebrovascular Disease and Their Influencing Factors
10.13359/j.cnki.gzxbtcm.2024.10.020
- VernacularTitle:脑血管疾病风险人群湿证相关的肠道菌群特征及影响因素研究
- Author:
Hai-Yan HUANG
1
,
2
,
3
;
Zhuo-Ran KUANG
;
Xiao-Jia NI
;
Qing SU
;
Miao-Miao MENG
;
Xiao-Bo YANG
;
Ye-Feng CAI
Author Information
1. 广东省中医院脑病中心,广东广州 510120
2. 广东省中医药科学院中医药防治脑血管病转化医学研究团队,广东广州 510120
3. 广州中医药大学第二临床医学院,广东广州 510120
- Keywords:
cerebrovascular disease;
stroke;
risk factors;
dampness syndrome;
gut flora;
atherosclerosis;
inflammatory response;
lipid metabolism
- From:
Journal of Guangzhou University of Traditional Chinese Medicine
2024;41(10):2636-2647
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the characteristics of gut flora related to dampness syndrome in the population at risk of cerebrovascular disease and to explore their influencing factors.Methods Based on the results of epidemiological investigation of damp syndrome in at-risk population of cerebrovascular disease in Guangdong from October 2021 to February 2023,60 subjects(including 41 at-risk cases of cerebrovascular disease and 19 healthy controls)were included in the study.The identification of dampness syndrome and the risk rating of stroke were carried out for the subjects,and fecal samples were collected.High-throughput 16S rRNA sequencing technology and bioinformatics methods were used to analyze the characteristics of gut flora.Results(1)A total of 53 cases(88.33％)were identified as dampness syndrome.There was significant difference in the quantitative score of dampness syndrome between the risk group and the healthy group,and between the low-,medium-and high-risk groups(P=0.016;P=0.041).(2)There was no statistical difference in the species and abundance of gut flora between the dampness syndrome group and the non-dampness syndrome group.(3)In the population identified as dampness syndrome,there was no significant difference in Alpha diversity between the healthy group and the risk group,but there was significant difference in Beta diversity analysis;LEfSe analysis found that Fusobacterium and Lactobacillus were enriched in the risk group;correlation analysis showed that the differential bacteria were related to the three risk factors of diabetes,dyslipidemia and obesity and carotid intima-media thickness(IMT).(4)In the population identified as dampness syndrome and having the risk of cerebrovascular disease,there was no significant difference in Alpha diversity among three groups with different levels of risks,while significant difference in Beta diversity was observed;LEfSe analysis showed that Acidaminococcaceae,Phascolarctobacterium and Butyricimonas were enriched in the low-risk group,Veillonellaceae was enriched in the medium-risk group,and Ruminococcus 2 and Alloprevotella were enriched in the high-risk group;correlation analysis showed that the differential bacteria were associated with high-density lipoprotein cholesterol(HDL-C),low-density lipoprotein cholesterol(LDL-C),white blood cell count(WBC),and neutrophil count(NEUT).Conclusion In the Guangdong population predominated by dampness syndrome,the severity of dampness syndrome is related to the risk of stroke,and the specific flora associated with sub-clinical atherosclerosis,inflammatory response and lipid metabolism are presented.
