Study on the Substance Benchmark Characteristic Chromatogram and Multi-Component Content Determination of Classical Formula Fuzi Decoction
10.13359/j.cnki.gzxbtcm.2024.09.031
- VernacularTitle:经典名方附子汤物质基准特征图谱及多成分含量测定研究
- Author:
Zhen-Zhen PAN
1
;
Yu-Jie XU
;
Jia-Hui LIU
;
Hui-Min YE
;
Zhen-Hua WANG
;
Chang-Qing LI
Author Information
1. 广州中医药大学青蒿研究中心,广东广州 510405
- Keywords:
Fuzi Decoction;
characteristic chromatogram;
multi-index content determination;
dry extract rate;
quality control
- From:
Journal of Guangzhou University of Traditional Chinese Medicine
2024;41(9):2442-2451
- CountryChina
- Language:Chinese
-
Abstract:
Objective To establish a high performance liquid chromatography(HPLC)characteristic chromatogram and multi-component content determination method for the substance benchmark of Fuzi Decoction.Methods The characteristic chromatogram method of Fuzi Decoction substance benchmark was established,and the Similarity Evaluation System of Chromatographic Fingerprint of Traditional Chinese Medicine(2012 Edition)was used to analyze the characteristic chromatograms of 15 batches of Fuzi Decoction substance benchmark samples.The HPLC content determination methods of 1 1 components in the Fuzi Decoction substance benchmark samples were established respectively,and the dry extract rate of 15 batches of Fuzi Decoction substance benchmark samples was determined.Results The similarity of characteristic chromatograms of 15 batches of Fuzi Decoction substance benchmark samples was greater than 0.9,and 12 common peaks were selected and eight of them were identified.The results showed that the contents of benzoylmesaconine,benzoylhypaconine,atractylenolide Ⅲ,ginsenoside Rg1,ginsenoside Re,ginsenoside Rb1,gallic acid,albiflorin,paeoniflorin,1,2,3,4,6-penta-galloyl glucose and benzoyl paeoniflorin were 0.050 3-0.191 1,0.026 7-0.047 0,0.043 0-0.127 6,0.554 6-1.006 8,0.568 7-0.979 5,0.929 9-1.726 1,1.058 9-2.118 4,1.430 3-4.965 5,6.882 9-9.511 1,0.056 1-0.262 5,0.160 6-0.369 0 mg/g,respectively.The average dry extract rate of the 15 batches of Fuzi Decoction substance benchmark samples was 29.54％.Conclusion The established characteristic chromatogram and multi-index content determination method are accurate and stable,which provides a basis for the quality control of the substance benchmark and related preparations of Fuzi Decoction.
