Effect of'Wenyang Tongmai'Moxibustion on the Inflammatory Response of ApoE-/-Atherosclerotic Mice Based on the SIRT1/FOXO3a Signaling Pathway
10.13359/j.cnki.gzxbtcm.2024.09.027
- VernacularTitle:基于SIRT1/FOXO3a信号通路探讨"温阳通脉"灸法对ApoE-/-动脉粥样硬化小鼠炎症反应的影响
- Author:
Jiao-Jiao XIONG
1
;
Xian-Ming WU
;
Chao-Bo YAN
;
Ning ZHANG
;
Zhou ZHU
;
Li PAN
;
Xiao-Fang YANG
Author Information
1. 贵州中医药大学,贵州贵阳 550025
- Keywords:
moxibustion;
Wenyang Tongmai;
atherosclerosis;
inflammatory factor;
silent message regulator 1(SIRT1);
forkhead box transcription factor O3a(FOXO3a);
mice
- From:
Journal of Guangzhou University of Traditional Chinese Medicine
2024;41(9):2410-2417
- CountryChina
- Language:Chinese
-
Abstract:
Objective To explore the mechanism of'Wenyang Tongmai'(warming yang to unblock meridians)moxibustion in preventing and treating atherosclerosis.Methods Ten C57BL/6J mice fed with normal diet were set as blank group.Thirty ApoE-/-mice were fed with high-fat diet to establish atherosclerosis model,and were randomly divided into model group,Simvastatin group and moxibustion group,with 10 mice in each group.The intervention began on the first day of modeling.The mice in the moxibustion group were given moxibustion at Danzhong(RN17),Shenque(RN8),Neiguan(PC6),Xuehai(SP10)points,and the Simvastatin group was given Simvastatin distilled water suspension by gavage for 12 weeks.After administration,the pathological structure of thoracic aorta in mice was observed by hematoxylin-eosin(HE)staining method.The ultrastructure of thoracic aortic endothelial cells in mice was observed by transmission electron microscopy.The levels of serum tumor necrosis factor α(TNF-α),intercellular adhesion molecule 1(ICAM-1)and vascular cell adhesion molecule 1(VCAM-1)in mice were detected by enzyme-linked immunosorbent assay(ELISA).The mRNA expression levels of silent information regulator 1(SIRT1)and forkhead box O3a(FOXO3a)in thoracic aorta were detected by real-time quantitative polymerase chain reaction(qRT-PCR).The protein expression levels of SIRT1 and FOXO3a in thoracic aorta were detected by Western Blot.Results Compared with the blank group,the pathological changes of thoracic aorta and vascular endothelial cells in the model group were obvious,the levels of serum inflammatory factor TNF-α,ICAM-1 and VCAM-1 were increased(P＜0.05 or P＜0.01),the mRNA and protein expressions of SIRT1 in thoracic aorta were decreased(P＜0.01),and the mRNA and protein expressions of FOXO3a had no significant difference(P＞0.05).Compared with the model group,the thoracic aorta and vascular endothelial cell structure of the mice in the Simvastatin group and the moxibustion group were obviously improved,the levels of serum TNF-α,ICAM-1 and VCAM-1 were decreased(P＜0.05),the mRNA and protein expressions of SIRT1 in the thoracic aorta were increased(P＜0.05 or P＜0.01),and the mRNA and protein expressions of FOXO3a had no significant difference(P＞0.05).There was no significant difference in the above indexes between the Simvastatin group and the moxibustion group(P＞0.05).Conclusion'Wenyang Tongmai'moxibustion can prevent and treat atherosclerotic in rats via regulating and controlling SIRT1/FOXO3a signaling pathway to reduce inflammatory response.
