Differential Analysis of Gut Flora in Patients with Osteoporosis of Different Traditional Chinese Medicine Syndrome Types
10.13359/j.cnki.gzxbtcm.2024.09.010
- VernacularTitle:不同中医证型骨质疏松症患者肠道菌群的差异性分析
- Author:
Zhi-Cheng ZHOU
1
;
Han TIAN
;
Xu-Rui ZHUANG
;
Zu-Jian LIANG
Author Information
1. 广州中医药大学,广东广州 510405
- Keywords:
osteoporosis;
gut flora;
traditional Chinese medicine(TCM)syndrome types;
spleen and kidney yang deficiency;
liver and kidney yin deficiency;
kidney deficiency and blood stasis;
microbiota
- From:
Journal of Guangzhou University of Traditional Chinese Medicine
2024;41(9):2289-2295
- CountryChina
- Language:Chinese
-
Abstract:
Objective To analyze the characteristics and differences of gut flora in patients with osteoporosis of different traditional Chinese medicine(TCM)syndrome types,thus to find out new therapeutic targets for the treatment of osteoporosis.Methods The clinical data and fecal samples of 57 patients with osteoporosis recruited in the Department of Osteoporosis,the Third Affiliated Hospital of Guangzhou University of Chinese Medicine from February 2023 to November 2023 were collected.The patients were differentiated as the TCM syndrome types of spleen and kidney yang deficiency group(22 cases),liver and kidney yin deficiency group(18 cases)and kidney deficiency and blood stasis group(7 cases).The differences in the structure and abundance of gut flora in patients with the above three TCM syndrome types were analyzed by16S rDNA sequencing technique.Results The results of Alpha(α)diversity showed that there were no significant differences in species richness,evenness and diversity of gut flora among the three groups(P＞0.05).Beta(β)diversity analysis showed that there were significant differences in gut flora among the three groups(P=0.011).At the phylum level,Bacteroidota,Firmicutes and Proteobacteria were the top three kinds of gut flora with the highest proportion in the three groups.At the genus level,the relative abundance of Bacteroides,Escherichia-Shigella,and Faecalibacterium was higher in the group of liver and kidney yin deficiency and in the group of kidney deficiency and blood stasis,while the group of spleen and kidney yang deficiency has a large proportion of other bacteria.Conclusion There exist differences in the composition of gut flora among patients with different TCM syndrome types,and the differences are shown at the level of phylum and genus.The results indicate that gut flora may be the targets in the TCM prevention and treatment of osteoporosis.
