Effect of Herbal Cake-Separated Moxibustion on the Expression of PI3K/AKT/mTOR in Atherosclerotic Rabbits
10.13359/j.cnki.gzxbtcm.2024.07.027
- VernacularTitle:隔药饼灸对动脉粥样硬化兔PI3K/AKT/mTOR表达的影响
- Author:
Han PENG
1
;
Hong-Fen YI
;
Xin-Yu CHEN
;
Qian LI
;
Meng-Xia XIAO
;
Hong-Hua LIU
;
Mai-Lan LIU
Author Information
1. 湖南中医药大学针灸推拿与康复学院,湖南长沙 410208
- Keywords:
herbal cake-separated moxibustion;
atherosclerosis;
PI3K/AKT/mTOR pathway;
inflammation;
rabbits
- From:
Journal of Guangzhou University of Traditional Chinese Medicine
2024;41(7):1839-1844
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the anti-atherosclerotic mechanism of herbal cake-separated moxibustion.Methods Twenty-four New Zealand purebred male rabbits were randomly divided into normal group,model group,herbal cake-separated moxibustion group and Atorvastatin group,with 6 rabbits in each group.Except for the normal group,the rabbits in the other groups were fed with high-fat diet mixed with Propylthiouracil(PTU)method to construct an atherosclerosis model.At the same period of modeling,corresponding intervention was given.After 12 weeks,the levels of serum total cholesterol(TC),triglyceride(TG)and low density lipoprotein cholesterol(LDL-C)were detected.The pathological changes of rabbit aortic tissue were observed by hematoxylin-eosin(HE)staining.The levels of serum tumor necrosis factor α(TNF-α),interleukin 6(IL-6)and interleukin 10(IL-10)were detected by enzyme-linked immunosorbent assay(ELISA).The contents of phosphatidylinositol 3-kinase(PI3K),phosphorylated protein kinase B(p-AKT)and phosphorylated mammalian target of rapamycin(p-mTOR)in myocardial tissue were detected by Western Blot.Results Compared with the normal group,the serum levels of TC,TG and LDL-C in the model group were significantly increased(P＜0.001),the aortic vascular endothelial structure was significantly damaged,the serum levels of TNF-α and IL-6 were increased(P＜0.001),the serum level of IL-10 was decreased(P＜0.01),and the protein expression levels of PI3K,p-AKT and p-mTOR in myocardial tissue were significantly decreased(P＜0.05 or P＜0.01).Compared with the model group,the above indexes in the herbal cake-separated moxibustion group and the Atorvastatin group were significantly improved(P＜0.05 or P＜0.01 or P＜0.001).Compared with the Atorvastatin group,the contents of TG and LDL-C in the herbal cake-separated moxibustion group were increased(P＜0.01),and other indicators did not change significantly(P＞0.05).Conclusion The mechanism of anti-atherosclerosis of herbal cake-separated moxibustion may be related to the activation of PI3K/AKT/mTOR signaling pathway and the regulation of the expressions of inflammatory mediators TNF-α,IL-6 and IL-10.
