Qidan Granules Improves Insulin Resistance of Adipocytes by Promoting CTRP3 to Activate PI3K/AKT Signaling Pathway
10.13359/j.cnki.gzxbtcm.2024.06.031
- VernacularTitle:芪丹颗粒通过促进CTRP3激活PI3K/AKT信号通路改善脂肪细胞胰岛素抵抗
- Author:
Yue ZHANG
1
;
Xu-Xi GUO
;
Ren-Qun YE
Author Information
1. 深圳市宝安区中医院内分泌科,广东深圳 518100
- Keywords:
Qidan granules;
type 2 diabetes mellitus(T2DM);
insulin resistance;
C1q/TNF-related protein 3(CTRP3);
PI3K/AKT signaling pathway;
inflammation;
3T3-L1 adipocytes
- From:
Journal of Guangzhou University of Traditional Chinese Medicine
2024;41(6):1581-1588
- CountryChina
- Language:Chinese
-
Abstract:
Objective To explore the mechanism of Qidan Granules prevent insulin resistance(IR)in adipocytes.Methods The IR model of 3T3-L1 adipocytes was induced by palmitic acid culture method.The blank control group,model group,Qidan Granules low-,medium-and high-dose groups,Qidan Granules+C1q/TNF-related protein 3(CTRP3)siRNA group were set up.Determination of glucose consumption in cell supernatant was performed;the levels of tumor necrosis factor α(TNF-α)and interleukin 6(IL-6)in the cell supernatant were determined by enzyme-linked immunosorbent assay(ELISA).Real-time quantitative polymerase chain reaction(qRT-PCR)was used to detect the gene expressions of CTRP3,TNF-α,IL-6 and glucose transporter 4(GLUT4).The protein expressions and phosphorylation levels of CTRP3,phosphoinositide-3 kinase(PI3K)and protein kinase B(AKT)were detected by Western Blot.Results Compared with the model group,the glucose consumption was increased and tended to 70％-82％of the blank control group,TNF-α content was decreased by 25％-45％,IL-6 content was decreased by 40％-55％,GLUT4 mRNA expression level was increased by 50％,and mRNA and protein expression levels of CTRP3 were increased by 25％-40％in the medium-and high-dose groups of Qidan Granules(all P＜0.05).Compared with the Qidan Granules group,the glucose consumption and GLUT4 mRNA expression level in the Qidan Granules+CTRP3 siRNA group were decreased by 33％and 27％,respectively,the contents of IL-6 and TNF-α were increased by 42％and 41％,respectively,and the protein expressions of p-PI3K and p-AKT were increased by 190％and 180％,respectively(all P＜0.05).Conclusion Qidan Granules may improve the insulin sensitivity of IR 3T3-L1 adipocytes and improve IR of adipocytes by promoting CTRP3 expression to reduce inflammatory response and activate PI3K/AKT signaling pathway.
