Effect of Shentong Zhuyu Granules on Inflammation and JAK/STAT Signaling Pathway in Rats with Rheumatoid Arthritis
10.13359/j.cnki.gzxbtcm.2024.06.029
- VernacularTitle:身痛逐瘀颗粒对类风湿性关节炎大鼠炎症及JAK/STAT信号通路的影响
- Author:
Yu-Chen XIAO
1
;
Qian-Qian MIAO
;
Long WANG
;
Xiao-Jun LIU
;
Lu ZHU
Author Information
1. 西安新润药业有限公司药物研究中心,陕西西安 710075
- Keywords:
Shentong Zhuyu Granules;
rheumatoid arthritis;
inflammatory factors;
JAK/STAT signaling pathway;
rats
- From:
Journal of Guangzhou University of Traditional Chinese Medicine
2024;41(6):1567-1573
- CountryChina
- Language:Chinese
-
Abstract:
Objective To observe the therapeutic effect and mechanism of Shentong Zhuyu Granules on rheumatoid arthritis(RA)rats.Methods There were 12 rats randomly selected from 60 rats as the normal group,and the remaining 48 rats were gaven two immunization in the simulated environment of'wind,cold and dampness'to construct the RA model.All 48 successfully modeled rats were randomly divided into model group,Shentong Zhuyu Granules high-,medium-and low-dose groups,with 12 rats in each group.All the groups were given corresponding administration for 21 days.Hematoxylin-eosin(HE)staining was used to observe and score the histopathology of synovial membrane of ankle joint in rats after administration.The swelling degree of bilateral ankle joints and feet of rats was compared before and after administration.Enzyme-linked immunosorbent assay(ELISA)was used to detect the contents of inflammatory factors tumor necrosis factor(TNF)-α,interleukin(IL)-1β,IL-6 and interferon(IFN)-γ in ankle joint synovial tissue of rats after administration.The protein expressions of Janus kinase(JAK)/signal transducer and activator of transcription(STAT)pathway in synovial tissues of rats were detected by Western Blot.Results Compared with the normal group,the synovial membrane of the rats in the model group proliferated,the structure of chondrocytes was disordered,and a large number of inflammatory cells infiltrated in the cartilage cavity,the scores of inflammatory cell infiltration and bone destruction were significantly increased(P＜0.05),the swelling degree of bilateral ankle joints and feet was increased(P＜0.05),the contents of TNF-α,IL-1β,IL-6 and IFN-γ in the synovial tissue,and the protein expression levels of JAK1,STAT3,phosphorylated STAT3(p-STAT3),STAT5 and phosphorylated STAT5(p-STAT5)were significantly increased(P＜0.05).Compared with the model group,the above indexes in the high-,medium-and low-dose groups of Shentong Zhuyu Granules were significantly improved(P＜0.05).Conclusion Shentong Zhuyu Granules can effectively improve RA in rats,and its mechanism is related to the inhibition of inflammatory factors and the activation of JAK/STAT signaling pathway.
