Genetic engineering drives the breakthrough of pig models in liver disease research
10.1016/j.livres.2024.09.003
- Author:
Xu CHENHAO
1
;
Fang XIXI
;
Xu XIAO
;
Wei XUYONG
Author Information
1. Zhejiang University School of Medicine
- Keywords:
Animal model;
Liver disease;
Genetic engineering;
CRISPR/Cas9;
Xenogeneic liver transplantation;
Pig
- From:
Liver Research
2024;8(3):131-140
- CountryChina
- Language:Chinese
-
Abstract:
Compared with the widely used rodents,pigs are anatomically,physiologically,and genetically more similar to humans,making them high-quality models for the study of liver diseases.Here,we review the latest research progress on pigs as a model of human liver disease,including methods for establishing them and their advantages in studying cystic fibrosis liver disease,acute liver failure,liver regeneration,non-alcoholic fatty liver disease,liver tumors,and xenotransplantation.We also emphasize the impor-tance of genetic engineering techniques,mainly the CRISPR/Cas9 system,which has greatly enhanced the utility of porcine models as a tool for substantially advancing liver disease research.Genetic engineering is expected to propel the pig as one of the irreplaceable animal models for future biomedical research.
