Effects of Sanghuang Tongxie Prescription on mitophagy in hippocampus of diabetic depression rats with depression
10.3760/cma.j.cn115398-20240410-00114
- VernacularTitle:桑黄通泻方对糖尿病伴抑郁状态大鼠海马线粒体自噬的影响
- Author:
Jingjing PU
1
;
Hong CHANG
;
Meng WANG
;
Ji'an LI
Author Information
1. 华北理工大学中医学院，唐山 063210
- Keywords:
Diabetes mellitus;
Depression;
Sanghuang Tongxie Formula;
Hippocampus;
Mitophagy;
Rats
- From:
International Journal of Traditional Chinese Medicine
2024;46(11):1439-1443
- CountryChina
- Language:Chinese
-
Abstract:
Objective:To observe the effect of Sanghuang Tongxie Prescription on mitophagy in hippocampus of diabetic depression rats.Methods:Totally 32 SD rats were divided into control group, model group, metformin group and Sanghuang Tongxie Prescription group according to random number table method, with 8 rats in each group. The model of diabetic depression rats with depression was established by streptozotocin (STZ), high-fat diet and chronic unpredictable mild stress except control group. Metformin group was given metformin 100 mg/kg, Sanghuang Tongxie Prescription group was given Sanghuang Tongxie Prescription suspension 5.7 g/kg, control group and model group were given the same volume of normal saline by gavage, once a day for 12 weeks. After administration, fasting plasma glucose (FPG), 2-hour postprandial blood glucose (2 hPG) and sucrose preference rate were measured after treatment; the expressions of PINK-1, Parkin, P62, Beclin-1 and LC3 2/1 were detected by Western blot.Results:Compared with the model group, the expressions of FPG and PG2h in Sanghuang Tongxie Prescription group decreased ( P<0.01), and the sucrose preference rate increased ( P<0.01); the expressions of PINK-1, Parkin, P62, Beclin-1 and LC3 2/1 in hippocampus decreased ( P<0.01). Conclusions:Diabetic rats are accompanied by varying degrees of pleasure loss and depressive state. Sanghuang Tongxie Prescription can improve the depressive state of rats. Its mechanism may be related to down-regulating the level of mitochondrial autophagy in the hippocampus and improving mitochondrial function.
