Tangeretin attenuating inflammatory and oxidative stress injury via Nrf2/NQO1 pathway in rats with spinal cord injury
10.3760/cma.j.cn115398-20240124-00309
- VernacularTitle:桔皮素调控Nrf2/NQO1通路减轻脊髓损伤大鼠炎症与氧化应激损伤
- Author:
Jianglin WU
1
;
Ming GAO
;
Chaolun LIANG
;
Kai WANG
;
Junqiang XIAO
;
Jiachang LIANG
;
Yan LIN
Author Information
1. 广州中医药大学第二附属医院骨伤科，广州 510120
- Keywords:
Spinal cord injury;
Tangeretin;
Inflammation;
Oxidative stress;
Nrf2/NQO1 signaling pathway;
Rats
- From:
International Journal of Traditional Chinese Medicine
2024;46(11):1462-1468
- CountryChina
- Language:Chinese
-
Abstract:
Objective:To explore the repairing effect and mechanism of tangeretin in rats with spinal cord injury.Methods:The rats were divided into sham-operation group, model group and tangeretin group according to random number table, with 8 rats in each group. Except for the sham-operation group, Allen hit method was used to make rat models in the other groups. After the model was successfully established, the tangeretin group was intragastrically administered with tangeretin 50 mg/kg, and the sham-operation group and the model group were intragastrically administered with an equal volume of normal saline once a day for 14 days. On days 0, 3, 7, and 14 after modeling, the motor function recovery of rats was assessed using the Basso-Beattie-Bresnahan (BBB) score; the morphological changes of the spinal cord tissues were observed using HE staining and Nissl staining; the SOD and GSH activities and MDA, IL-1β, TNF-α, and IL-10 levels in the spinal cord tissues of rats in each group were measured using ELISA kit detection; the GFAP and Neun expressions in the spinal cord tissues were detected by immunofluorescence; the IL-1β, TNF-α, IL-10, nuclear factor E2-related factor 2 (Nrf-2), and NAD (P) H-quinone oxidoreductase 1 (NQO-1) expressions in the spinal cord tissues were detected by Western blot.Results:Compared with the model group, the BBB score increased in the tangeretin group ( P<0.05), HE staining score decreased ( P<0.05), and the number of Nissl bodies increased ( P<0.05); the level of IL-10, SOD and GSH activities increased ( P<0.05), and IL-1β, TNF-α and MDA levels decreased in the spinal cord tissue ( P<0.05); GFAP fluorescence intensity decreased ( P<0.05) and NeuN fluorescence intensity increased ( P<0.05); the relative expression of IL-1β and TNF-α decreased ( P<0.05), and the relative expressions of IL-10, Nrf-2 and NQO-1 protein increased ( P<0.05). Conclusions:Tangeretin can exert anti-inflammatory and anti-oxidative stress effects through the Nrf2/NQO1 signaling pathway and alleviate early spinal cord injury in rats. On the other hand, it may promote the recovery of spinal cord injury by reducing glial scar generation and promoting neural cellogenesis.
