Effects of Hejie Jusan Decotion on NF-κB/NLRP3/Caspase-1 cell pyroptosis pathway in kidney of rats with diabetic nephropathies
10.3760/cma.j.cn115398-20231207-00071
- VernacularTitle:和解聚散方对糖尿病肾病大鼠肾脏NF-κB/NLRP3/Caspase-1细胞焦亡通路的影响
- Author:
Jieqiong YAO
1
;
Zhaoli CUI
Author Information
1. 山西省中医院（山西省中医药研究院）肾病一科，太原 030012
- Keywords:
Diabetes nephropathy;
Pyroptosis;
Hejie Jusan Decotion;
NF-κB/NLRP3/Caspase-1 pathway;
Rats
- From:
International Journal of Traditional Chinese Medicine
2024;46(11):1450-1454
- CountryChina
- Language:Chinese
-
Abstract:
Objective:To explore the effect of Hejie Jusan Decoction on pyroptosis in diabetic nephropathies (DN) rats; To explore its mechanism based on the NF-κB/nucleotide binding oligomeric domain like receptor protein 3 (NLRP3)/Caspase-1 signal pathway.Methods:Totally 60 SD rats were divided into blank group, model group, potassium losartan group, and Hejie Jusan Decoction high-, medium-, and low-dosage groups. In addition to the blank group, the other five groups were established DN rat models. Hejie Jusan Decoction high-, medium-, and low-dosage groups were given 0.5, 1.0, and 2.0 g/ml concentrations of Hejie Jusan Decoction, respectively; the potassium losartan group was orally administered with 20 mg/kg potassium losartan; The blank group and model group were given equal volumes of physiological saline by gavage, once a day, for consecutive 28 days. After medication, the levels of serum creatinine (SCr), urea nitrogen (BUN), and 24-hour urine protein (24 hUP) were detected; pathological changes in renal tissue were detected by HE staining; Western blot method was used to detect the expressions of P65, NLRP3, Caspase-1, and GSDMD protein in renal tissues.Results:Compared with the model group, the SCr, BUN, and 24-hUP of rats in the Hejie Jusan Decoction high-dosage group and the potassium losartan group decreased ( P<0.01). The expressions of P65, NLRP3, Caspase-1, and GSDMD protein in renal tissues of Hejie Jusan Decoction groups and potassium losartan group decreased ( P<0.05 or P<0.01). Conclusion:Hejie Jusan Decotion may affect pyroptosis by inhibiting the activation of NF-κB/NLRP3/Caspase-1 pathway, reducing renal function damage and pathological changes, so as to achieve the effect of treating diabetes nephropathy.
