Simultaneous determination of the contents of eight components in Guanxin Danshen Tablets by HPLC-DAD-ELSD
10.3760/cma.j.cn115398-20240220-00098
- VernacularTitle:HPLC-DAD-ELSD法同步测定冠心丹参片中8个成分含量
- Author:
Yougen WU
1
;
Yanjun CHEN
Author Information
1. 江西应用科技学院现代管理学院，南昌 330100
- Keywords:
Guan Xin Dan Shen Pian;
HPLC-DAD-ELSD;
Content determination;
Quality control
- From:
International Journal of Traditional Chinese Medicine
2024;46(11):1480-1484
- CountryChina
- Language:Chinese
-
Abstract:
Objective:To establish an method for the simultaneous determination of the contents of Sodium salvianolic acid, protocatechualdehyde, salvianolic acid B, tanshinone Ⅱ A, Panax notoginseng saponin R 1, ginsenoside Rg 1, ginsenoside Re, and ginsenoside Rb1 in Guanxin Danshen Tablets. Method:HPLC-DAD-ELSD method was used, and the chromatographic column was Thermo Hypersil Keystone C18 column (4.6 mm×250 mm,5 μm); mobile phase was acetonitrile(A)-0.03 mol/L ammonium acetate (containing formic acid, pH 2.4)(B), gradient elution; flow rate was 1.0 ml/min; column temperature was 25 ℃, and DAD detection wavelength was 280 nm, and injection amount was 20 μl. ELSD setting parameters: drift tube temperature: 113 ℃, carrier gas flow rate: 3.1 L/min.Results:Sodium salvianolic acid, protocatechualdehyde, salvianolic acid B, tanshinone Ⅱ A, Panax notoginseng saponin R 1, ginsenoside Rg 1, ginsenoside Re, and ginsenoside Rb 1 were tested at injection rates of 3.075 0-18.450 0, 0.040 0-0.240 0, 0.800 0-4.800 0, 0.060 5-0.363 0, 0.716 0-4.296 0, 0.105 6-0.633 0, 0.605 5-3.633 0, 0.697 5-4.185 0 μg, respectively. The linear relationship between the peak area and the g range was good, with r all within the range of 0.996 4 to 0.999 8. The recovery rates ( n=6) of the added samples were 101.70%, 96.85%, 104.20%, 99.63%, 102.60%, 100.80%, 99.71%, and 101.20%, respectively. The RSDs were 1.88%, 3.13%, 1.93%, 1.41%, 1.71%, 1.88%, 3.24%, and 2.11%, respectively. Conclusion:The determination method established in this article has good specificity, precision, repeatability, and stability, and is suitable for the determination of 8 components in Guanxin Danshen Tablets, providing reference for improving the quality control of Guanxin Danshen Tablets.
