Study on fingerprints of commercial Shenling Baizhu Powder and Shenling Baizhu Pills
10.3760/cma.j.cn115398-20240108-00090
- VernacularTitle:市售参苓白术散和参苓白术丸指纹图谱研究
- Author:
Tanrong JIA
1
;
Ping YU
;
Jiajia WANG
;
Yifan HU
;
Runpeng CHAI
;
Chengze DONG
;
Yinying BA
Author Information
1. 首都医科大学中医药学院，北京 100069
- Keywords:
Shen Ling Bai Zhu San;
Shen ling bai zhu pill;
HPLC fingerprint;
similarity;
Quality control
- From:
International Journal of Traditional Chinese Medicine
2024;46(10):1338-1342
- CountryChina
- Language:Chinese
-
Abstract:
Objective:To establish an HPLC fingerprint of Shenling Baizhu Powder and Shenling Baizhu Pills; To evaluate the quality consistency of commercial Shenling Baizhu Powder and Shenling Baizhu Pills.Methods:HPLC method was adopted with Agilent TC-C18 column (250 mm×4.6 mm, 5 μm). The mobile phase was 0.05% phosphoric acid-acetonitrile with gradient elution; the flow rate was 1.0 ml/min; the detection wavelength was 203 nm; the column temperature was 25 ℃. The HPLC fingerprints of Shenling Baizhu Powder and Shenling Baizhu Pills from different manufacturing enterprises were established and analyzed by using the Similarity Evaluation System of Chromatographic Fingerprint of TCM (Version 2012) software for similarity evaluation, and the main chromatographic peaks were identified.Results:The control fingerprints of Shenling Baizhu Powder and Shenling Baizhu Pills were obtained. 73 common peaks and 79 common peaks were identified respectively. The similar degrees of all samples were over 0.92. The quality consistency of drugs different batches of different production enterprises was good. A total of 10 components were identified, including Liquiritin, Ginsenoside Rg1, Ginsenoside Rb1, Glycyrrhizin, Isoliquiritin, Ginsenoside Rd, Glycyrrhizic acid, AtractylenolideⅠ, AtractylenolideⅡ and AtractylenolideⅢ.Conclusions:The established HPLC fingerprints can quickly evaluate the formulation quality of Shenling Baizhu Powder and Shenling Baizhu Pills, providing basis the quality control.
