Effects of Buzhong Yiqi Decoction on the expressions of MRP, LRP and P-gp in transplantation tumor of A549 tumor-bearing nude mice
10.3760/cma.j.cn115398-20231008-00036
- VernacularTitle:补中益气汤对A549细胞荷瘤裸鼠移植瘤MRP、LRP、P-gp表达的影响
- Author:
Yuetong LIU
1
;
Qirui MU
;
He LI
;
Jingyi HUANG
;
Yuan GAO
Author Information
1. 辽宁中医药大学病理教研室，沈阳 110847
- Keywords:
Bu Zhong Yi Qi Tang;
Lung cancer;
Multidrug resistance-associated proteins;
Lung resistance protein;
P-glycoprotein;
A549 cells
- From:
International Journal of Traditional Chinese Medicine
2024;46(9):1145-1149
- CountryChina
- Language:Chinese
-
Abstract:
Objective:To investigate the effects of Buzhong Yiqi Decoction on the expressions of multidrug resistance-related protein (MRP), lung resistance-related protein (LRP), and P-glycoprotein (P-gp) in transplantation tumor of A549 tumor-bearing nude mice.Methods:A549 cells were cultured to establish xenograft models of tumor-bearing nude mice. According to the random number table method, they were divided into blank control group, cisplatin group, cisplatin + Buzhong Yiqi Decoction low-dosage group, cisplatin + Buzhong Yiqi Decoction medium-dosage group, cisplatin + Buzhong Yiqi Decoction high-dosage group. After 25 days of corresponding drug intervention, the mRNA expressions of MRP, LRP and P-gp in transplantation tumors were detected by Real-Time PCR, and Western blot was used to detect the expressions of MRP, LRP and P-gp proteins in xenograft tissues in each group.Results:Compared with blank control group, the mRNA and protein expressions of MRP, LRP and P-gp in cisplatin group were significantly down-regulated ( P<0.05). Compared with cisplatin group, the mRNA and protein expressions of MRP, LRP and P-gp in the cisplatin + Buzhong Yiqi Decoction low-dosage group, cisplatin + Buzhong Yiqi Decoction medium-dosage group, cisplatin+Buzhong Yiqi Decoction high-dosage group decreased ( P<0.05). Conclusion:Buzhong Yiqi Decoction can inhibit the expressions of MRP, LRP and P-gp in transplantation tumor of A549 tumor-bearing nude mice and without dosage dependence.
