Effects of Quyu Jiedu Decoction on free iron and NF-κB, IL-6 and TNF-α in ectopic tissues of rats with endometriosis
10.3760/cma.j.cn115398-20231008-00046
- VernacularTitle:祛瘀解毒方对子宫内膜异位症大鼠异位组织游离铁及NF-κB、IL-6、TNF-α的影响
- Author:
Yiming LIU
1
;
Xiaoliang LIU
;
Zhihong LIU
;
Fang LIAN
Author Information
1. 山东中医药大学第一临床医学院，济南 250000
- Keywords:
Endometriosis;
Inflammation;
Free iron;
Quyu Jiedu Decoction;
Rats
- From:
International Journal of Traditional Chinese Medicine
2024;46(9):1150-1154
- CountryChina
- Language:Chinese
-
Abstract:
Objective:To investigate the effects of Quyu Jiedu Decoction on the levels of free iron and NF-κB, IL-6 and TNF-α in ectopic tissues of rats with endometriosis.Methods:Totally 18 rats were divided into blank group, model group and Quyu Jiedu Decoction group according to the random number table method, with 6 rats in each group. Except for the blank group, the endometriosis model was prepared by autologous transplantation in the rest of the groups. Quyu Jiedu Decoction was given 13.23 g/kg of Quyu Jiedu Decoction, and the rats in the blank group and model group were gavaged with the same volume of normal saline for 4 consecutive weeks for consecutive 4 weeks. The pathological changes of endometriosis tissues of the three groups were observed by HE staining; the free iron content of the ectopic tissues of the rats was detected by ferrous ion colorimetric test box; ELISA was used to detect the levels of IL-6 and TNF-α; the expressions of NF-κB, IL-6 and TNF-α mRNA of rat ectopic tissues were detected by q-PCR.Results:The results of HE staining showed that there was more inflammatory cell infiltration in ectopic lesions in the model group, and the inflammatory cell infiltration of ectopic lesions in the Quyu Jiedu Decoction group was significantly improved. Compared with the model group, the content of free iron in ectopic tissues decreased ( P<0.05), the levels of IL-6 and TNF-α decreased ( P<0.05), and the mRNA expressions of NF-κB and TNF-α were down-regulated ( P<0.05). Conclusion:Quyu Jiedu Decoction can improve the expressions of free iron, IL-6, TNF-α and NF-κB in ectopic tissues of rats with endometriosis, and has a certain effect on alleviating the inflammatory state of ectopic tissues in rats with endometriosis.
