Establishment of HPLC chromatogram and content determination of 8 nucleoside components of Aspongopus
10.3760/cma.j.cn115398-20231205-00040
- VernacularTitle:九香虫HPLC特征图谱构建和8个核苷类成分含量测定
- Author:
Shuangyan TANG
1
;
Jiabao WEI
;
Mingli MA
;
Weizhi ZHAO
;
Suqin CAI
;
Hui ZHANG
Author Information
1. 华润三九现代中药制药有限公司，惠州 516000
- Keywords:
Aspongopus;
High-performance liquid chromatography;
Assay (TCD);
Characteristic map;
Quality control
- From:
International Journal of Traditional Chinese Medicine
2024;46(9):1193-1198
- CountryChina
- Language:Chinese
-
Abstract:
Objective:To establish HPLC chromatogram for Aspongopus; To determine 8 nucleoside components of uracil, adenine, uridine, uric acid, hypoxanthine, adenosine, xanthine and canine quinolinic acid; To provide reference for quality control and evaluation.Methods:The Agilent ZORBAX SB-Aq chromatographic column (4.6 mm×250 mm, 5 μm) was used for gradient elution with mobile phases consisting of a methanol (A) and 0.05% phosphoric acid (B). The column temperature was 25 ℃, the flow rate was 0.8 ml/min, and the detection wavelength was 254 nm. HPLC chromatograms for Aspongopus were established and the contents of 8 components were determined.Results:The characteristic chromatogram of 15 batches aspongopus herbs was established. A total of 10 common characteristic peaks were identified and 8 were identified. The similarity between the characteristic chromatogram of samples and the control chromatogram was 0.969-0.997. The content determination showed that the linear range of uracil, adenine, urin, uric acid, hypoxanthine, adenosine, xanthine and xanuric acid was among 0.002 0-0.644 0, 0.001 4-0.448 0, 0.001 0-1.257 0, 0.005 4-6.221 0, 0.001 0-0.724 0, 0.001 0-0.644 0, 0.002 0-1.113 0, 0.003 8-2.059 0 μg, respectively, with a good linear relationship ( r≥0.999); the repeatability and stability of RSD were <2.0%, and the average sampling recovery rate was between 99.36% and 103.40%. Conclusion:The characteristic chromatogram and content determination method established in this study are simple, reliable, reproducible and accurate, and can be used for the qualitative and quantitative analysis of Aspongopus and can provide a reference for the quality evaluation method of the Aspongopus.
