Study on the quality of the Dryopteridis Crassirhizomatis Rhizoma from different producing areas
10.3760/cma.j.cn115398-20231011-00081
- VernacularTitle:不同产地绵马贯众药材质量研究
- Author:
Siqiong CAO
1
;
Yunjing QIU
;
Jiaying HE
;
Chunlin ZHONG
;
Min XU
Author Information
1. 广东一方制药有限公司 广东省中药配方颗粒企业重点实验室，佛山 528200
- Keywords:
Dryopteridis Crassirhizomatis Rhizoma;
Specific chromatogram;
Assay (TCD);
Quality control
- From:
International Journal of Traditional Chinese Medicine
2024;46(9):1187-1192
- CountryChina
- Language:Chinese
-
Abstract:
Objective:To establish UPLC specific chromatogram method and content determination method of Dryopteridis Crassirhizomatis Rhizoma; To comprehensively evaluate the Dryopteridis Crassirhizomatis Rhizoma from different producing areas.Methods:UPLC method was used to establish the specific chromatogram of 15 batches of Dryopteridis Crassirhizomatis Rhizoma from different producing areas. The quality was evaluated by cluster analysis and partial least square analysis. The content of total flavonoids was determined by ultraviolet spectrophotometry.Results:The established specific chromatogram identified a total of 21 common peaks, and identified 4 components through comparison with control samples. The clustering and partial least squares analysis showed that the samples from Anshan City in Liaoning Province, Yanbian Korean Autonomous Prefecture in Jilin Province, Jiamusi City in Heilongjiang Province, Jilin City in Jilin Province had a certain consistency. The results of total flavone content determination showed that the content of samples from different producing areas was different.Conclusion:The established UPLC characteristic chromatogram and content determination method of total flavone content of Dryopteridis Crassirhizomatis Rhizoma can reflect the quality of Dryopteridis Crassirhizomatis Rhizoma in different places. The quality of samples from different origins is similar, but the contents of the three producing areas fluctuated greatly.
